Fcgr2b^{em1(FCGR2B)Synbl}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fcgr2b^{em1(FCGR2B)Synbl}
• Name: Fc receptor, IgG, low affinity IIb; endonuclease-mediated mutation 1, Synbal
• MGI ID: MGI:7607009
• Synonyms: Humanized Mouse-hFcgr2b
• Gene: Fcgr2b Location: Chr1:170786186-170804116 bp, - strand Genetic Position: Chr1, 78.02 cM
• Alliance: Fcgr2b^{em1(FCGR2B)Synbl} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Fcgr2b^{em1(FCGR2B)Synbl} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FCGR2B (2213)

  Fcgr2b

  (MGI:95499)

• Mutation details: Using a CRISPR/Cas9 approach, an active genetic cassette (AGC) was used to remove the endogenous mouse gene coding region and replace it with a functional human FCGR2B coding region under the control of the endogenous mouse promoter. The AGC included a Kozak consensus translational initiation sequence and human FCGR2B cDNA mini-gene cassette (HsFCGR2B), 1101 bp native human FCGR2B3UTR (HsFCGR2B 3UTR) U6 promoter, gRNA, Stop-FRT cassette, and 3HR. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Fcgr2b Mutation: 48 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)