Igs7^{tm4(tetO-Rpl10*/GCaMP8s,CAG-tTA2)Genie}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm4(tetO-Rpl10*/GCaMP8s,CAG-tTA2)Genie}
• Name: intergenic site 7; targeted mutation 4, Janelia GENIE Project Team
• MGI ID: MGI:7607114
• Synonyms: TIGRE2-RiboL1-jGCaMP8s-IRES-tTA2
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm4(tetO-Rpl10*/GCaMP8s,CAG-tTA2)Genie} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inducible, Reporter, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The transgenic construct contains an HS4 insulator-Tet response element/promoter (pTRE2)-loxP-Stop-loxP-RiboL1-CaMP8s-WPRE-pA-HS4 insulator-pCAG-lox2272-Stop-lox2272-FLAG-IRES-tTA2-pA cassette introduced to the Igs7 intergenic region. RiboL1 is a ribo-tag sequence modified from Rpl10, ribosomal protein L10. gCaMP8s (sensitive) incorporates a hexa-histidine affinity tag, an endothelial nitric oxide synthase peptide (ENOSP; modified with N19T, S24I, and S26M), a circularly permutated green fluorescent protein (cpGFP; modified with Q88E), the rat calcium-binding protein calmodulin (CaM; modified with F286Y and Q315H), and LKI amino acid linkers that replace the RS20 (previously referred to as M13) linkers. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 66 strains or lines available

Notes

ES cell = made from Ai99 Flp-in mouse (derived from G4)

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)