Actbem1(tetO-EGFP)Kta
Endonuclease-mediated Allele Detail
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Symbol: |
Actbem1(tetO-EGFP)Kta |
Name: |
actin, beta; endonuclease-mediated mutation 1, Kohichi Tanaka |
MGI ID: |
MGI:7610673 |
Synonyms: |
Actb-tetO-FLEX-EGFP |
Gene: |
Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
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Alliance: |
Actbem1(tetO-EGFP)Kta page
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Strain of Origin: |
Not Applicable
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Allele Type: |
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Endonuclease-mediated (Conditional ready, Inducible, Reporter) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Mutation details: CRISPR-targeting inserted a tetracycline response element fused to a minimal cytomegalovirus (CMV) promoter driving inducible and conditional expression of inverted EGFP flanked by two pairs of loxP and lox2722 (FLEX switch) and polyadenylation sequence.
(J:345651)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Actb Mutation: |
51 strains or lines available
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Original: |
J:345651 Aida T, et al., Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice. Genome Biol. 2015 Apr 29;16:87 |
All: |
2 reference(s) |
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