Cdc27^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: Cdc27^tm1.1Ics
• Name: cell division cycle 27; targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:7616486
• Synonyms: Cdc27^S92F
• Gene: Cdc27 Location: Chr11:104393571-104441446 bp, - strand Genetic Position: Chr11, 67.79 cM
• Alliance: Cdc27^tm1.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342743
• Parent Cell Line: BD10 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: Serine codon 93 (TCT) in exon 4 was changed to phenylalanine (TTT) (p.S93F). A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and second loxP site were inserted into intron 5. The neo cassette was removed through subsequent Flp-mediated recombination, leaving exons 4-5 floxed. Mutations in the human ortholog are associated with non-syndromic intellectual disability. (J:342743)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdc27 Mutation: 56 strains or lines available

References

• Original: J:342743 Meziane H, et al., Large-Scale Functional Assessment of Genes Involved in Rare Diseases with Intellectual Disabilities Unravels Unique Developmental and Behaviour Profiles in Mouse Models. Biomedicines. 2022 Dec 6;10(12)
• All: 1 reference(s)