Cers2tm2Kwi
Targeted Allele Detail
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Symbol: |
Cers2tm2Kwi |
Name: |
ceramide synthase 2; targeted mutation 2, Klaus Willecke |
MGI ID: |
MGI:7621228 |
Synonyms: |
CerS2 FloxNeo (Del) |
Gene: |
Cers2 Location: Chr3:95222102-95230910 bp, + strand Genetic Position: Chr3, 40.74 cM, cytoband F2
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Alliance: |
Cers2tm2Kwi page
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Allele Type: |
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Targeted (Conditional ready) |
Mutation: |
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Insertion
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Mutation details: A loxP site, a copy of the wild type genomic sequence containing exons 3-11 including the stop codon and poly(A) signal sequences, an FRT site flanked neomycin resistance gene cassette and a second loxP site were inserted into intron 2. Codons 79P-120R in endogenous exons 3 and 4 were deleted and IRES sequences and the EGFP reporter gene were inserted into the 3' UTR between stop codon and poly(A) signal. This allele expresses the wild type peptide. Only after Cre-mediated deletion of wild type exons 3-11 will it express the mutated peptide and the EGFP reporter.
(J:265853)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Cers2 Mutation: |
60 strains or lines available
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Original: |
J:265853 Bickert A, et al., Inactivation of ceramide synthase 2 catalytic activity in mice affects transcription of genes involved in lipid metabolism and cell division. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Jul;1863(7):734-749 |
All: |
1 reference(s) |
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