Tet2^em1Mmda
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tet2^em1Mmda
• Name: tet methylcytosine dioxygenase 2; endonuclease-mediated mutation 1, Meelad M Dawlaty
• MGI ID: MGI:7623879
• Synonyms: Tet2^m
• Gene: Tet2 Location: Chr3:133169438-133250882 bp, - strand Genetic Position: Chr3, 61.84 cM
• Alliance: Tet2^em1Mmda page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280583
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Histidine codon 1295 (CAC) in exon 9 was changed to tyrosine (TAC) (ENSMUSP00000096203:p.H1295Y) and aspartic acid codon 1297 (GAC) was changed to alanine (GCC) (ENSMUSP00000096203:p.D1297A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. These mutations are in the Fe2+ binding pocket of the encoded protein and cause the loss of its catalytic activity. (J:280583)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tet2 Mutation: 778 strains or lines available

References

• Original: J:280583 Ito K, et al., Non-catalytic Roles of Tet2 Are Essential to Regulate Hematopoietic Stem and Progenitor Cell Homeostasis. Cell Rep. 2019 Sep 3;28(10):2480-2490.e4
• All: 2 reference(s)