Rev1^em2Gpc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rev1^em2Gpc
• Name: REV1, DNA directed polymerase; endonuclease-mediated mutation 2, Gerry P Crossan
• MGI ID: MGI:7643779
• Synonyms: Rev1^AA
• Gene: Rev1 Location: Chr1:38091867-38168882 bp, - strand Genetic Position: Chr1, 16.46 cM, cytoband B
• Alliance: Rev1^em2Gpc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Aspartic acid codon 568 (GAT) and glutamic acid codon 569 (GAA) in exon 11 were changed to alanine (GCG, GCA) (p.DE568_569delinsAA) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutations render the encoded peptide catalytically inactive. (J:348075)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rev1 Mutation: 68 strains or lines available

References

• Original: J:348075 Shah P, et al., Primordial germ cell DNA demethylation and development require DNA translesion synthesis. Nat Commun. 2024 May 3;15(1):3734
• All: 1 reference(s)