Gt(ROSA)26Sor^{tm6(Zap70,-EGFP)Ics}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm6(Zap70,-EGFP)Ics}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 6, Mouse Clinical Institute
• MGI ID: MGI:7780009
• Synonyms: GT(ROSA)26Sor^{tm1(Zap70, IRES-EGFP)Ics}, Rosa26^LSL-Zap70
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm6(Zap70,-EGFP)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:307147
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm6(Zap70,-EGFP)Ics} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Zap70 (MGI:99613)	conditionally expressed

• Mutation details: The following elements were inserted into an intron: an adenovirus splice acceptor site, a conditional STOP cassette (loxP site flanked cassette consisting of PGK promoter, neomycin resistance gene, PGK p(A) and 3X SV40 p(A) signal sequences), followed by mouse Zap70 cDNA, an FRT site flanked IRES-EGFP cassette and bovine GH p(A) signal sequences. This allele only expresses Zap70 and EGFP after Cre-mediated removal of the stop cassette. (J:307147)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1027 strains or lines available

References

• Original: J:307147 Sadras T, et al., Developmental partitioning of SYK and ZAP70 prevents autoimmunity and cancer. Mol Cell. 2021 May 20;81(10):2094-2111.e9
• All: 1 reference(s)