Btg3^em1(IMPC)Ics
Endonuclease-mediated Allele Detail

Summary

• Symbol: Btg3^em1(IMPC)Ics
• Name: BTG anti-proliferation factor 3; endonuclease-mediated mutation 1, Mouse Clinical Institute
• MGI ID: MGI:7781822
• Gene: Btg3 Location: Chr16:78156748-78174069 bp, - strand Genetic Position: Chr16, 45.36 cM
• Alliance: Btg3^em1(IMPC)Ics page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at the Institut Clinique de la Souris by electroporating Cas9 protein and 4 sgRNAs (equivalent to CTCTCATAAATGAGGAGACC, ATGAGCCCAGAGGAATCACC,TGGCTGGAGAAGCTGGCCGA and CAGGAGGGCAGCGCTTCCTT), resulting in a 4891 bp deletion that includes exons 3 and 4 (ENSMUSE00000482894 and ENSMUSE00000476530; GRCm39), leading to a frameshift and premature stop codon. (J:358405)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Btg3 Mutation: 19 strains or lines available

References

• Original: J:358405 MGI and ICS/MCI, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC) Generated by ICS/MCI. Database Release. 2024
• All: 1 reference(s)