Phenotypes Associated with This Genotype

Genotype
MGI:2450865

• Allelic Composition: Tsg101^tm1Mak/Tsg101^tm1Mak
• Genetic Background: either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:67553 )

• mutant embryos fail to develop past E6.5

• by E8.5, most embryos are either in resorption or degenerating within the yolk sac

embryo

absent inner cell mass proliferation ( J:67553 )

• in vitro, E3.5 mutant blastocysts fail to show ICM proliferation after 6 days in culture

abnormal gastrulation ( J:67553 )

embryonic growth arrest ( J:67553 )

• mutant embryos fail to progress significantly beyond E5.5 due to a dramatic accumulation of Trp53 protein

decreased embryo size ( J:67553 )

• at E5.5 and esp. at E6.5, mutant embryos are significantly smaller than wild-type embryos

absent mesoderm ( J:67553 )

• at E6.5 , mutant embryos show absence of a developing mesoderm

failure of primitive streak formation ( J:67553 )

• at E6.5, mutant embryos show absence of a primitive streak

abnormal embryonic-extraembryonic boundary morphology ( J:67553 )

• at E6.5, homozygotes exhibit a poorly defined boundary between the embryonic and extraembryonic regions

abnormal parietal endoderm morphology ( J:67553 )

• at E6.5, mutant embryos display a poorly defined parietal endoderm

disorganized extraembryonic tissue ( J:67553 )

• at E5.5, mutant embryos display a poorly defined extraembryonic region

• by E6.5, the extraembryonic region appears severely underdeveloped and disorganized

abnormal visceral endoderm morphology ( J:67553 )

• at E5.5, mutant embryos display a disorganized visceral endoderm

growth/size/body

decreased embryo size ( J:67553 )

• at E5.5 and esp. at E6.5, mutant embryos are significantly smaller than wild-type embryos

cellular

decreased mitotic index ( J:67553 )

• at E6.5, mutant embryos exhibit a significantly lower mitotic index than wild-type embryos (0.2-0.3 versus 0.7-0.8, respectively)

decreased cell proliferation ( J:67553 )

• at E6.5, mutant embryos display a significantly reduced cellular proliferation relative to wild-type embryos, as shown by a >40% decrease in BrdU-positive nuclei

absent inner cell mass proliferation ( J:67553 )

• in vitro, E3.5 mutant blastocysts fail to show ICM proliferation after 6 days in culture