Analysis Tools
behavior/neurological
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• following an injection of a hypertonic solution, mice drink water less than similarly treated wild-type mice
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• reduced thermal hypersensitvitiy following inflammation
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• mutants show normal responses to noxious mechanical stimuli but exhibit no vanilloid-evoked pain behavior, are impaired in the detection of painful heat, and show little thermal hypersensitivity in the setting of inflammation
(J:61534)
• cheek injection of capsaicin does not evoke pain behavior (forepaw facial wiping), in contrast to robust facial wiping observed in treated wild-type
(J:197482)
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• mutants exhibit no vanilloid (capsaicin and resiniferatoxin) evoked pain behavior: no behavioral response and less swelling with vanilloid injection into plantar skin of hind paw, no aversive response to drinking capsaicin supplemented water, and no reduction in temperature in response to capsaicin injection
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• mutants exhibit reduced sensitivity to painful heat in behavioral tests, with longer mean withdrawal latencies in the tail immersion test at temperatures greater than 48 degrees Celcius and longer response latencies at temperatures greater than 50 degrees Celcius in the hot plate assay
(J:61534)
• mutants treated with mustard oil show little or no change in hot plate latency
(J:61534)
• mice show increased latencies (impaired nociceptive response) in tail-immersion and hot plate paradigms
(J:197482)
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nervous system
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• in response to osmotic stimulation, organum vasculosum lamina terminalis neurons fail to exhibit changes in membrane potential or conductance unlike similarly treated wild-type cells
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• in response to osmotic stimulation, organum vasculosum lamina terminalis neurons fail to exhibit an increase in action potential firing compared with similarly treated wild-type cells
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• significant attenuation of oxidated IP defensin 1 (IP-O) induced Ca2+ signaling in dorsal root ganglion (DRG) neurons
• reduction in the proportion of HIS-sensitive cells in DRG
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• sensory neurons and primary afferent fibers show a reduction in proton (pH5) sensitivity in vitro (acid-evoked nociception)
• incidence of noxious heat-evoked currents of the moderate-threshold (greater than 43 degrees Celcius) class is reduced in cultured sensory neurons or sensory nerve fibers but high-threshold responses remain intact
• vanilloid compounds, capsaicin and resiniferatoxin, are completely inactive on neurons from mutants, showing no increases in calcium or inward currents
• mutants treated with mustard oil show little or no chmutants treated with mustard oil show no enhancement of thermally evoked responses in wide dynamic range neurons of the lumbar dorsal horn
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adipose tissue
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• when fed a high fat diet compared with similarly treated wild-type mice
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• when fed a high fat diet compared with similarly treated wild-type mice
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• when fed a high fat diet compared with similarly treated wild-type mice
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growth/size/body
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• when fed a high fat diet compared with similarly treated wild-type mice
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homeostasis/metabolism
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• when fed a high fat diet compared with similarly treated wild-type mice
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• whether fed a standard or high fat diet, cold-treated mice do not exhibit the drop in body temperature observed in similarly treated wild-type mice
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liver/biliary system
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• when fed a high fat diet, mice exhibit reduced hepatic fat content and reduced lipid droplets compared with similarly treated wild-type mice
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integument
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• when fed a high fat diet compared with similarly treated wild-type mice
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• decreased IP-O induced scratch response
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