Analysis Tools
hematopoietic system
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• homozygotes displayed normal bleeding times
• at 8-12 weeks of age, homozygotes had normal platelet numbers in peripheral blood
• early events of platelet activation (e.g. shape change and granule release) and the initial rate of platelet aggregation appeared normal
• no differences were detected in the cleavage pattern and kinetics of calpain substrates
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• platelets showed impaired tyrosine phosphorylation of several proteins upon activation with either calcium ionophore or thrombin, including the beta3 subunit of alphaIIb-beta3 integrin (~70% reduction)
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• platelet aggregation was decreased by 50-60% in response to thrombin (10 nM), ADP (20 uM), collagen (20 ug/ml), and calcium ionophore A23187 (1.0 uM)
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• subtle differences were detected in the ATP secretion from dense granules upon thrombin and collagen activation; no differences were detected in the ADP- and calcium ionophore A23187-treated platelets
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homeostasis/metabolism
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• platelets were defective in retracting clots, especially when clot formation was induced with 1.0 nM thrombin
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• platelets showed impaired tyrosine phosphorylation of several proteins upon activation with either calcium ionophore or thrombin, including the beta3 subunit of alphaIIb-beta3 integrin (~70% reduction)
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• platelet aggregation was decreased by 50-60% in response to thrombin (10 nM), ADP (20 uM), collagen (20 ug/ml), and calcium ionophore A23187 (1.0 uM)
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• subtle differences were detected in the ATP secretion from dense granules upon thrombin and collagen activation; no differences were detected in the ADP- and calcium ionophore A23187-treated platelets
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