Analysis Tools
immune system
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• at 7-10 wks of age, homozygotes display no differences in T cell development or homeostasis of peripheral T cell subsets relative to wild-type control littermates
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• in response to TCR/CD3-mediated signals delivered by anti-CD3 mAb, homozygotes display up to 50% reduction in T cell proliferation relative to wild-type mice
• co-stimulation with anti-CD28 partially compensates for the severely reduced proliferative response to TCR/CD3-mediated T cell activation, suggesting that mutant T cells are capable of responding to CD28-mediated co-stimulatory signals
• no significant differences in T cell proliferation are observed in response to stimulation with PMA plus ionomycin
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• in response to stimulation with soluble anti-CD3 (1 g/ml), mutant splenocytes show a severe reduction in IFN-gamma production, along with a >90% decrease in IFN-gamma mRNA levels, as revealed by densitometry
• however, IFN-gamma production is restored when mutant T cells are co-stimulated with anti-CD28
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• in response to stimulation with soluble anti-CD3 (1 g/ml), mutant splenocytes show a severe reduction in the supernatant levels of IL-2 (as determined by ELISA) along with a ~80% reduction in IL-2 mRNA levels (as determined by densitometry) relative to similarly activated wild-type T cells
• a significant reduction in supernatant IL-2 levels and relative IL-2 mRNA levels is also observed when mutant T cells are co-stimulated with anti-CD3 and anti-CD28
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• in response to co-stimulation with anti-CD3 and anti-CD28, mutant splenocytes show an increase in IL-4 production relative to similarly activated wild-type splenocytes
• however, no significant differences in IL-4 production are observed when mutant T cells are stimulated with anti-CD3 alone
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hematopoietic system
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• in response to TCR/CD3-mediated signals delivered by anti-CD3 mAb, homozygotes display up to 50% reduction in T cell proliferation relative to wild-type mice
• co-stimulation with anti-CD28 partially compensates for the severely reduced proliferative response to TCR/CD3-mediated T cell activation, suggesting that mutant T cells are capable of responding to CD28-mediated co-stimulatory signals
• no significant differences in T cell proliferation are observed in response to stimulation with PMA plus ionomycin
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cellular
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• in response to TCR/CD3-mediated signals delivered by anti-CD3 mAb, homozygotes display up to 50% reduction in T cell proliferation relative to wild-type mice
• co-stimulation with anti-CD28 partially compensates for the severely reduced proliferative response to TCR/CD3-mediated T cell activation, suggesting that mutant T cells are capable of responding to CD28-mediated co-stimulatory signals
• no significant differences in T cell proliferation are observed in response to stimulation with PMA plus ionomycin
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