Phenotypes Associated with This Genotype

Genotype
MGI:3041947

• Allelic Composition: Prkcb^tm1Tara/Prkcb^tm1Tara
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased B cell proliferation ( J:34818 )

• after stimulation with antibody to IgM, compared to controls

• reduced basal proliferation in the absence of stimuli, compared to controls

• reduced proliferation in response to LPS

• combined treatment with antibody to CD40 and with IL-4 provoked equally strong proliferation, compared to controls

• combined treatment with phorbol ester, PdBU, and calcium ionophore, ionomycin, provoked equally strong proliferation, compared to controls

decreased B cell number ( J:34818 )

• although B cells were present in lymph nodes and spleen, absolute numbers of splenic B cells were reduced compared to wild-type controls

• normal frequency and absolute number of pre-B (IgM-, B220lo, CD43-) and mature B cells (IgM+, B220hi) in bone marrow

decreased B-1 B cell number ( J:34818 )

• reduced numbers of B-1 (CD23-, IgM+) cells in peritoneal cavity

decreased B-1a cell number ( J:34818 )

• reduced numbers of B-1a (CD5+, IgM+) cells in peritoneal cavity

decreased IgG3 level ( J:34818 )

• IgG3 reduced

decreased IgM level ( J:34818 )

immune system

immune system phenotype ( J:34818 )

N • normal thymus size and cellularity, contained normal ratios of double positive (CD4+CD8+) and single positive (CD4+ or CD8+) cells

N • single positive (CD4+ or CD8+) T cells present in spleen and lymph nodes at frequency similar to wild-type controls

N • normal T cell proliferation

decreased B cell proliferation ( J:34818 )

• after stimulation with antibody to IgM, compared to controls

• reduced basal proliferation in the absence of stimuli, compared to controls

• reduced proliferation in response to LPS

• combined treatment with antibody to CD40 and with IL-4 provoked equally strong proliferation, compared to controls

• combined treatment with phorbol ester, PdBU, and calcium ionophore, ionomycin, provoked equally strong proliferation, compared to controls

decreased B cell number ( J:34818 )

• although B cells were present in lymph nodes and spleen, absolute numbers of splenic B cells were reduced compared to wild-type controls

• normal frequency and absolute number of pre-B (IgM-, B220lo, CD43-) and mature B cells (IgM+, B220hi) in bone marrow

decreased B-1 B cell number ( J:34818 )

• reduced numbers of B-1 (CD23-, IgM+) cells in peritoneal cavity

decreased B-1a cell number ( J:34818 )

• reduced numbers of B-1a (CD5+, IgM+) cells in peritoneal cavity

abnormal humoral immune response ( J:34818 )

• failed to mount detectable humoral response to T cell-independent antigen (NP-Ficoll), but not T cell-dependent antigen (NP-chicken gamma-globulin conjugate)

• reduced primary antibody responses to hapten (NP) and carrier (chicken gamma-globulin), compared to controls

• reduced secondary immune response to chicken gamma-globulin, but not hapten (NP), compared to controls

decreased IgG3 level ( J:34818 )

• IgG3 reduced

decreased IgM level ( J:34818 )

cellular

decreased B cell proliferation ( J:34818 )

• after stimulation with antibody to IgM, compared to controls

• reduced basal proliferation in the absence of stimuli, compared to controls

• reduced proliferation in response to LPS

• combined treatment with antibody to CD40 and with IL-4 provoked equally strong proliferation, compared to controls

• combined treatment with phorbol ester, PdBU, and calcium ionophore, ionomycin, provoked equally strong proliferation, compared to controls