Phenotypes Associated with This Genotype

Genotype
MGI:3525449

• Allelic Composition: Pde4b^tm1Mct/Pde4b^tm1Mct
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:106828 )

• following LPS-induced shock, mice exhibit less mortality (27% compared to 52%) and die later (3.5 days post-treatment compared to 1.5 days post-treatment) compared to wild-type mice

immune system

impaired neutrophil chemotaxis ( J:94853 )

• chemotaxis in response to KC and macrophage-inflammatory protein-2 was reduced in homozygous mutant neutrophils compared to wild-type

impaired neutrophil recruitment ( J:94853 )

• 31% reduction in the number of neutrophils that are recruited to the lung following lipopolysaccharide (LPS) inhalation compared to wild-type and homozygous Pde4a mutants

decreased tumor necrosis factor secretion ( J:106828 )

• LPS-stimulated TNF-alpha production is decreased to 43.5+/-3.5% of that in control mice

• thioglycollate-elicited macrophages produce 52.5+/-7.8% of TNF-alpha compared with wild-type cells

• macrophages do not respond to rolipram inhibition of LPS-stimulated TNF-alpha production

cellular

impaired neutrophil chemotaxis ( J:94853 )

• chemotaxis in response to KC and macrophage-inflammatory protein-2 was reduced in homozygous mutant neutrophils compared to wild-type

hematopoietic system

impaired neutrophil chemotaxis ( J:94853 )

• chemotaxis in response to KC and macrophage-inflammatory protein-2 was reduced in homozygous mutant neutrophils compared to wild-type

impaired neutrophil recruitment ( J:94853 )

• 31% reduction in the number of neutrophils that are recruited to the lung following lipopolysaccharide (LPS) inhalation compared to wild-type and homozygous Pde4a mutants