Phenotypes Associated with This Genotype

Genotype
MGI:3531461

• Allelic Composition: Scx^tm1Eno/Scx^tm1Eno; Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺
• Genetic Background: either: (involves: 129S4/SvJaeSor * 129S7/SvEvBrd * Swiss) or (involves: 129S4/SvJaeSor * 129S7/SvEvBrd * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between somite formation and embryo turning, incomplete penetrance ( J:55991 )

• by E8.5, homozygotes appear to resorb or degenerate and lack distinct features; no mutants are detected after E8.5

embryo

failure to gastrulate ( J:55991 )

• mutants are unable to show even the earliest signs of gastrulation

embryonic growth arrest ( J:55991 )

• homozygotes appear normal in size and morphology at the early egg cylinder stage (E6.0), but fail to develop beyond this stage

embryonic growth retardation ( J:55991 )

• at E6.5 and E7.5, homozygotes exhibit growth retardation relative to wild-type embryos

decreased egg cylinder size ( J:55991 )

• up to E6.0, mutant embryos appear normal and contain embryonic and extraembryonic structures and proamniotic cavities; however, by E6.5, the simple egg cylinder fails to elongate

• homozygotes show a rapid decline of proliferative activity and elevated cell death in the epiblast at the time of egg cylinder elongation; in contrast, proliferation occurs normally prior to E6.0

absent mesoderm ( J:55991 )

• mutant embryos fail to form a histologically recognizable mesoderm at gastrulation; they fail to form mesodermal cells or express mesodermal markers

failure of primitive streak formation ( J:55991 )

• mutant embryos contain abnormal pyknotic cells and fail to form a primitive streak

abnormal extraembryonic tissue morphology ( J:55991 )

• by E6.5, mutant extraembryonic regions appear to be underdeveloped relative to wild-type; a cavitated extraembryonic region fails to form

abnormal ectoplacental cone morphology ( J:55991 )

• mutant ectoplacental cones are proportionately correct in size but degenerate after E6.5

abnormal parietal endoderm morphology ( J:55991 )

• visceral and parietal endoderm layers are present at E6.5 and E7.5, but appear developmentally delayed and fail to form the flattened endodermal cell phenotype in the embryonic region

abnormal visceral endoderm morphology ( J:55991 )

• visceral and parietal endoderm layers are present at E6.5 and E7.5, but appear developmentally delayed and fail to form the flattened endodermal cell phenotype in the embryonic region

growth/size/body

embryonic growth retardation ( J:55991 )

• at E6.5 and E7.5, homozygotes exhibit growth retardation relative to wild-type embryos