Phenotypes Associated with This Genotype

Genotype
MGI:3583921

• Allelic Composition: Ptk2^tm1.1Guan/Ptk2^tm1.2Guan; Tg(Tek-cre)12Flv/0
• Genetic Background: involves: 129P2/OlaHsd * C3H * C57BL/6 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:99637 )

• mutant embryos are progressively lost starting at E13.5 with only 3% of pups at birth having the conditional null genotype

cardiovascular system

abnormal vascular endothelial cell morphology ( J:99637 )

• endothelial cells lining small capillaries have pyknotic and karyorrhetic changes and many are rounded, detached, and sloughing off into the lumen

• apoptotic and necrotic endothelial cells are seen

abnormal capillary morphology ( J:99637 )

• many capillaries with abnormal endothelial cells are collapsed

abnormal placenta vasculature ( J:99637 )

• arterial canals and canal branches lack fetal red blood cells and some of the vessels appear collapsed; however the heart appears normal

abnormal vascular branching morphogenesis ( J:99637 )

• at E13.5, the vascular network in the head is reduced and no clear outlines of viscera or axial skeleton with PECAM-1 staining

abnormal vitelline vasculature morphology ( J:99637 )

• fewer branched vessels and a marked decrease in sprouting angiogenesis are seen in the yolk sac

hemorrhage ( J:99637 )

• at E13.5, multifocal, scattered, and variably sized hemorrhages are seen and at E14.5 these are large and superficial

• at E13.5, the thickened dermis also has numerous perivascular hemorrhages

embryo

abnormal placenta vasculature ( J:99637 )

• arterial canals and canal branches lack fetal red blood cells and some of the vessels appear collapsed; however the heart appears normal

abnormal vitelline vasculature morphology ( J:99637 )

• fewer branched vessels and a marked decrease in sprouting angiogenesis are seen in the yolk sac

embryonic growth retardation ( J:99637 )

• seen in about 5% of embryos at E10.5

decreased embryo size ( J:99637 )

abnormal amnion morphology ( J:99637 )

• thickening of the amnion as a result of edema

• prominent amniotic blood vessels are not seen

thin placenta labyrinth ( J:99637 )

• the thickness of the placental labyrinth is reduced

homeostasis/metabolism

edema ( J:99637 )

• present in the membranes of the amnion at E13.5 and superficially at E14.5

growth/size/body

embryonic growth retardation ( J:99637 )

• seen in about 5% of embryos at E10.5

decreased embryo size ( J:99637 )

integument

thick dermal layer ( J:99637 )

• at E13.5, thickening and expansion of the dermis with loosely arranged mesenchymal cells mixed with acellular pale esosinophilic material