Phenotypes Associated with This Genotype

Genotype
MGI:3620576

• Allelic Composition: Itgam^tm1Myd/Itgam^tm1Myd
• Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

decreased apoptosis ( J:38052 )

• in cultured total leukocyte populations from harvested peritoneal exudates, extravasated null neutrophils show a 56% and 45% reduction in apoptosis after 6 and 8 hours in culture respectively, compared to wild-type neutrophils

abnormal leukocyte adhesion ( J:38052 )

• neutrophils from null animals are significantly impaired with respect to their ability to adhere to the endothelium in response to leukotriene B4

impaired neutrophil phagocytosis ( J:38052 )

• null neurophils isolated from peritoneal lavage of thioglycollate-treated mice are unable to phagocytose complement-opsonized particles; neutrophil spreading on glass is attenuated in null neutrophils; these neutrophils also show a 60% reduction in the oxidative burst associated with phagocytosis

hematopoietic system

abnormal leukocyte adhesion ( J:38052 )

• neutrophils from null animals are significantly impaired with respect to their ability to adhere to the endothelium in response to leukotriene B4

impaired neutrophil phagocytosis ( J:38052 )

• null neurophils isolated from peritoneal lavage of thioglycollate-treated mice are unable to phagocytose complement-opsonized particles; neutrophil spreading on glass is attenuated in null neutrophils; these neutrophils also show a 60% reduction in the oxidative burst associated with phagocytosis

increased eosinophil cell number ( J:38052 )

• mutants show increased eosinophil accumulation at 10.5 hours, 48 hours and 5 days in the model of peritonitis

increased neutrophil cell number ( J:38052 )

• null animals have 2-fold more neutrophils in the peritoneal exudates after 2 hours in an experimental model of peritonitis than did wild-type animals

immune system

abnormal leukocyte adhesion ( J:38052 )

• neutrophils from null animals are significantly impaired with respect to their ability to adhere to the endothelium in response to leukotriene B4

impaired neutrophil phagocytosis ( J:38052 )

• null neurophils isolated from peritoneal lavage of thioglycollate-treated mice are unable to phagocytose complement-opsonized particles; neutrophil spreading on glass is attenuated in null neutrophils; these neutrophils also show a 60% reduction in the oxidative burst associated with phagocytosis

increased eosinophil cell number ( J:38052 )

• mutants show increased eosinophil accumulation at 10.5 hours, 48 hours and 5 days in the model of peritonitis

increased neutrophil cell number ( J:38052 )

• null animals have 2-fold more neutrophils in the peritoneal exudates after 2 hours in an experimental model of peritonitis than did wild-type animals