Phenotypes Associated with This Genotype

Genotype
MGI:3656076

• Allelic Composition: Dag1^tm1Kcam/Dag1^tm1Kcam
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:40601 )

• most homozygotes are substantially resorbed by E10.5

embryo

failure to gastrulate ( J:40601 )

• homozygotes fail to gastrulate

embryonic growth arrest ( J:40601 )

• homozygotes fail to progress beyond the early egg cylinder stage of development

• however, early differentiation of extraembryonic lineages and implantation appear unaffected

decreased embryo size ( J:40601 )

• by E7.5, homozygous mutant embryos are much smaller than wild-type embryos

abnormal embryonic tissue morphology ( J:40601 )

• starting at E6.5, homozygotes typically have a significantly smaller embryonic region

• only a small mass of embryonic tissue is detected at E10.5

absent mesoderm ( J:40601 )

• at E7.5, homozygotes lack any identifiable mesodermal tissue

abnormal extraembryonic tissue morphology ( J:40601 )

absent extraembryonic ectoderm ( J:40601 )

• at E6.5, homozygotes lack any significant development of extraembryonic ectoderm

• in contrast, visceral endoderm and a well-formed pro-amniotic cavity are clearly evident

abnormal Reichert's membrane morphology ( J:40601 )

• at E6.5, homozygotes lack a continuous Reichert's membrane; maternal red blood cells are thus found in the yolk sac cavity as early as E5.5

• only occasional "patches" of laminin and collagen IV, two major structural elements of Reichert's membrane, are detected

• in contrast, the basement membrane between the visceral endoderm and ectoderm appears normal

• Reichert's membrane defects are not due to a lack of parietal endoderm

growth/size/body

decreased embryo size ( J:40601 )

• by E7.5, homozygous mutant embryos are much smaller than wild-type embryos