Phenotypes Associated with This Genotype

Genotype
MGI:3656142

• Allelic Composition: Arnt^tm1.1Gonz/Arnt^tm1.2Gonz; Tg(KRT5-cre)1Tak/0
• Genetic Background: involves: 129X1/SvJ * C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:85934 , J:86533 )

• mutants die within 24 hours after birth due to severe dehydration (J:85934)

• all die within 24 hours of birth (J:86533)

• application of a salve to prevent dehydration allows mice to survive longer than 24 hours after birth (J:86533)

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:85934 )

N • mutants (epithelial cell-specific Arnt deleted) exhibit normal susceptibility to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced thymic involution unlike mutants with T cell-specific Arnt deletion (Arnt^tm1.1Gonz/Arnt^tm1.2Gonz Tg(Lck-cre)I57Jxm/0) which are resistant to the effects of TCDD

dehydration ( J:85934 , J:86533 )

• rapid dehydration after birth (J:86533)

impaired skin barrier function ( J:86533 )

• seen at P0 and E17, particularly around the ventral neck and perineal areas

growth/size/body

weight loss ( J:86533 )

• rapid and steady weight loss after birth

• weight loss is decreased with application of a salve to prevent dehydration

integument

abnormal epidermis stratum corneum morphology ( J:86533 )

• tightly packed

decreased keratohyalin granule number ( J:86533 )

• keratohyalin granules appear sparse

abnormal skin physiology ( J:86533 )

• abnormal ceramide composition; however cholesterol and fatty acid content is similar to controls

increased keratinocyte apoptosis ( J:86533 )

impaired skin barrier function ( J:86533 )

• seen at P0 and E17, particularly around the ventral neck and perineal areas

cellular

increased keratinocyte apoptosis ( J:86533 )