Analysis Tools
immune system
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• mice show no signs of T cell activation, lymphadenopathy, or splenomegaly over 32 weeks; T cells are naive, with no expression of activation markers
• primary response to antigen stimulation in vitro is similar to Rag1-null, Tg(DO11.10)10Dlo T cells
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• during secondary response to low-dose antigen stimulation in vitro, T cells show 2.2-3 fold higher proliferation compared to Rag2-null, Tg(Do11.10)10Dlo mice
• transferred T cells are resistant to tolerance induction in vivo in hosts after a tolerizing dose of ovalbumin peptide; in vivo expansion of T cells 4 and 7 days after treatment is 2.2- and 1.4-fold greater than Rag2-null, transgenic mice
• T cells given a tolerogenic stimulus proliferate and secrete Il-2 robustly upon restimulation in contrast to poor response in Rag2-null, Tg(DO11.10)10Dlo mice
• T cells given a tolerogenic stimulus do not exhibit a blockade after entering G1 phase of cell cycle, and readily enter S phase, while Ctla4-sufficient mutant cells do not progress past G1
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• during secondary response, on days 2 and 3, T cells show increased production of interferon gamma and Il-4 than transgenic, Rag2-null mice
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hematopoietic system
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• during secondary response to low-dose antigen stimulation in vitro, T cells show 2.2-3 fold higher proliferation compared to Rag2-null, Tg(Do11.10)10Dlo mice
• transferred T cells are resistant to tolerance induction in vivo in hosts after a tolerizing dose of ovalbumin peptide; in vivo expansion of T cells 4 and 7 days after treatment is 2.2- and 1.4-fold greater than Rag2-null, transgenic mice
• T cells given a tolerogenic stimulus proliferate and secrete Il-2 robustly upon restimulation in contrast to poor response in Rag2-null, Tg(DO11.10)10Dlo mice
• T cells given a tolerogenic stimulus do not exhibit a blockade after entering G1 phase of cell cycle, and readily enter S phase, while Ctla4-sufficient mutant cells do not progress past G1
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