Phenotypes Associated with This Genotype

Genotype
MGI:3720100

• Allelic Composition: Lats2^tm1Noj/Lats2^tm1Noj
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Lats2^tm1Noj/Lats2^tm1Noj embryos exhibit severe nervous system developmental defects

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:123480 )

• while 30 embryos are recovered at E10.5, only 3 embryos are recovered at E11.5 and none are recovered at E12.5 or later

embryo

abnormal pharyngeal arch morphology ( J:123480 )

• at E13.5, branchial arches exhibit delayed development

embryonic growth retardation ( J:123480 )

• at E13.5, growth retardation is evident in the whole embryo and in a number of organs including branchial arches and skeletal muscle

decreased embryo size ( J:123480 )

cellular

abnormal intracellular organelle morphology ( J:123480 )

• 24% of mouse embryonic fibroblasts contain an increase in centrosome number (greater than 2 per cell) where 7.6% of wild-type cells have greater than 2 centrosomes

abnormal cell nucleus morphology ( J:123480 )

• 15.1% of mouse embryonic fibroblasts have an enlarged nucleus compared to only 1.7% of wild-type cells

increased cell nucleus count ( J:123480 )

• 3.4-fold more mouse fibroblast cells are multinucleated relative to wild-type cells

polyploidy ( J:123480 )

• some mouse embryonic fibroblast cells show polyploidy

abnormal mitosis ( J:123480 )

• the number mouse embryonic fibroblast cells that fail cytokinesis is increased 3.3-fold relative to wild-type cells

• mouse embryonic fibroblast cells exit mitosis faster than wild-type cells

increased spinal cord apoptosis ( J:123480 )

• at E13.5, apoptotic cells are observed in the spinal cord

nervous system

increased spinal cord apoptosis ( J:123480 )

• at E13.5, apoptotic cells are observed in the spinal cord

abnormal fourth ventricle morphology ( J:123480 )

• at E13.5, surviving mice have a reduced number of nerve cells in the neuroepithelial layer of the fourth ventricle as well as infiltration of inflammatory cells

• at E13.5, the neuroepithelial layer is thinner and contains many pyknotic cells

abnormal spinal cord morphology ( J:123480 )

• at E10.5 and E13.5, spinal cords have reduced number of nerve cells and the density of nerve fibers

muscle

delayed muscle development ( J:123480 )

• at E13.5, skeletal muscle development is delayed

growth/size/body

embryonic growth retardation ( J:123480 )

• at E13.5, growth retardation is evident in the whole embryo and in a number of organs including branchial arches and skeletal muscle

decreased embryo size ( J:123480 )

cardiovascular system

small heart ( J:123480 )

• at E13.5, surviving mice have small hearts with only modest developmental retardation

craniofacial

abnormal pharyngeal arch morphology ( J:123480 )

• at E13.5, branchial arches exhibit delayed development