Phenotypes for Rbl1 Tg(Pax6-cre,GFP)2Pgr MGI:3722928 MGI Mouse

abnormal optic nerve morphology ( J:124204 )

• the optic nerve is thin due to the loss of retinal ganglion cells

abnormal eye development ( J:124204 )

• retinal transition (RTC) undergo ectopic DNA synthesis and increased apoptosis

• at P8 or P18 when cell division is completed in the wild-type retina, ectopic RTC divisions are detected

abnormal retina morphology ( J:124204 )

• Slc18a3 staining of mature starburst amacrine cells (SACs) is absent from the peripheral retina

• apoptosis eliminates most retinal ganglion cells

decreased retina rod cell number ( J:124204 )

• apoptosis eliminates many rod cells

abnormal amacrine cell morphology ( J:124204 )

• starburst amacrine cells (SACs) have defects in differentiation not associated with cell cycle or apoptosis

• only 1 Calb2+ SAC track is detectable instead of 3 normally detected in the inner plexiform layer

• Slc18a3 staining of mature SACs is absent from the peripheral retina

• however, SAC survival and process outgrowth is normal

• as markers of differentiation are detected early in the cell body if at all, synthesis or stability and transport of SAC markers is defective

• 5.6% of Camk2+ SAC express Chat and Slc18a3 compared to 60% in wild-type mice, 3.7% in Rbl1/E2f1 null mice and 91% in Rbl1/E2f3 null mice

• apoptosis eliminates most bipolar cells

• after P8, mice exhibit a reduction in Callb2+ starburst amacrine ccell bodies, indicative of amacrine cells

• only 1 Calb2+ starburst amacrine cell track is detectable instead of 3 normally detected in the inner plexiform layer

thin retina outer nuclear layer ( J:124204 )

• the outer nuclear layer is thin due to the loss of rods

abnormal cone electrophysiology ( J:124204 )

• light-adapted (photopic) response is defective

abnormal rod electrophysiology ( J:124204 )

• the response to dim light in dark-adapted (scotopic) conditions is defective