Analysis Tools
growth/size/body
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• mice are ~20% heavier than wild-type or Selltm1Tft mutant mice
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immune system
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• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 92% compared to wild-type
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• tumor necrosis factor alpha (Tnfa) treatment reduces leukocyte rolling flux below level seen in Selltm1Tft mutant mice
• rolling velocities of leukocytes are significantly greater at times <30 minutes after surgery compared to Selltm1Tft mutant mice (40 vs 19 um/second)
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• increased significantly compared to wild-type, comparable to Icam1-deficient mice
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• number of circulating neutrophils is increased over wild-type (580% of wild-type number)
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• number of circulating lymphocytes is increased over wild-type (200% of wild-type number)
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• number of circulating monocytes is increased over wild-type (640% of wild-type number)
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• mast cell infiltration of tissue after wounding is lower in mutants with or without Sele/Selp blockade at 3 and 7 days compared to wild-type animals; bFGF or PDGF signifcantly increase mast cell infiltration at 3 and 7 days in mutants with Sele/Selp blockade
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• macrophage infiltration is reduced at 3 and 7 days after wounding in mutants with or without Sele/Selp blockade relative to wild-type; numbers are reduced to greater degree with Sele/Selp blockade in mutants at 3 days but difference is not significant at 7 days
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• numbers in tissue after wounding are lower in mutants compared to controls
• bFGF or PDGFsignifcantly increase macrophage infiltration at 3 and 7 days in mutants with Sele/Selp blockade
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• after wounding, levels of cytokine mRNAs including TNFalpha, Il6, Il10, and TGFbeta are all reduced in mutants with or without Sele/Selp blockade compared to controls
• bFGF or PDGF significantly increases CD3+ T cell infiltration at 3 and 7 days in mutants with Sele/Selp blockade
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hematopoietic system
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• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 92% compared to wild-type
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• tumor necrosis factor alpha (Tnfa) treatment reduces leukocyte rolling flux below level seen in Selltm1Tft mutant mice
• rolling velocities of leukocytes are significantly greater at times <30 minutes after surgery compared to Selltm1Tft mutant mice (40 vs 19 um/second)
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• increased significantly compared to wild-type, comparable to Icam1-deficient mice
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• number of circulating neutrophils is increased over wild-type (580% of wild-type number)
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• number of circulating lymphocytes is increased over wild-type (200% of wild-type number)
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• number of circulating monocytes is increased over wild-type (640% of wild-type number)
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• mast cell infiltration of tissue after wounding is lower in mutants with or without Sele/Selp blockade at 3 and 7 days compared to wild-type animals; bFGF or PDGF signifcantly increase mast cell infiltration at 3 and 7 days in mutants with Sele/Selp blockade
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• macrophage infiltration is reduced at 3 and 7 days after wounding in mutants with or without Sele/Selp blockade relative to wild-type; numbers are reduced to greater degree with Sele/Selp blockade in mutants at 3 days but difference is not significant at 7 days
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• numbers in tissue after wounding are lower in mutants compared to controls
• bFGF or PDGFsignifcantly increase macrophage infiltration at 3 and 7 days in mutants with Sele/Selp blockade
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neoplasm
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• when injected with melanoma cells, mice exhibit a 2.3 fold increase in tumor volume on day 7 compared to wild-type mice; difference in tumor volume is 5.8-fold compared to wild-type on day 14
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cardiovascular system
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• at 3 and 7 days following injury, vascular density in the wound bed in mutants with or without Sele and Selp blockade is less than that observed in wild-type controls; treatment with bFGF increases vascular vessel density at 3 days after wounding in mutants with Sele/Selp blockade, but this is not maintained at 7 days
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homeostasis/metabolism
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• excisional wound healing is impaired in mutant mice and mutants or wild-type mice with Sele and Selp inhibition by monoclonal antibodies compared to wild-type mice at 3 and 7 days after wounding
• open wound area at 3 and 7 days in mutants with or without antibody treatment is significantly larger than in untreated wild-type mice
• granulation tissue formation at 3 days after wounding is significantly less than controls; formation at 7 days is less than in wild-type with Sele and Selp blockade or mutants with Sele and Selp blockade; treatment of mutants with Sele/Selp blockade with bFGF and to a lesser extent with PDGF normalizes granulation tissue formation at 7 days after wounding
• addition of basic FGF (bFGF) significantly reduces open wound area at 7 days after wounding in mutants with Sele/Selp blockade
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integument
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• keratinocyte migration in wound healing is inhibited in mutants at 3 and 7 days compared to wild-type mice, and is further inhibited by Sele and Selp blockade; treatment with bFGF almost normalized keratinocyte migration in wounds by day 7 in mutants with Sele/Selp blockade
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cellular
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• numbers in tissue after wounding are lower in mutants compared to controls
• bFGF or PDGFsignifcantly increase macrophage infiltration at 3 and 7 days in mutants with Sele/Selp blockade
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• keratinocyte migration in wound healing is inhibited in mutants at 3 and 7 days compared to wild-type mice, and is further inhibited by Sele and Selp blockade; treatment with bFGF almost normalized keratinocyte migration in wounds by day 7 in mutants with Sele/Selp blockade
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• 2 hours after thioglycollate-induced peritonitis, neutrophil entry into peritoneum is significantly inhibited by 92% compared to wild-type
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• tumor necrosis factor alpha (Tnfa) treatment reduces leukocyte rolling flux below level seen in Selltm1Tft mutant mice
• rolling velocities of leukocytes are significantly greater at times <30 minutes after surgery compared to Selltm1Tft mutant mice (40 vs 19 um/second)
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