Analysis Tools
hematopoietic system
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• platelets from chimeric mice containing bone marrow with the conditional knockout express 15% less alphaIIbeta3 integrin on their surface
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• platelets fail to form lamellipodia and spread out on a fibrinogen coated surface when exposed to low levels of thrombin
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• conditional knockout platelets fail to aggregate in vitro to high concentrations of thrombin, ADP, collagen, and other platelet agonists
• platelets fail to attach to a collagen coated surface even under low shear stress compared to control platelets that cover 53% of the surface area
• platelets fail to bind fibrinogen as measured by flow cytometry
• degranulation of mutant platelets to low levels of agonist is half that of chimeric controls
• Mn2+ addition enables platelets to bind fibrinogen
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homeostasis/metabolism
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• conditional knockout platelets fail to aggregate in vitro to high concentrations of thrombin, ADP, collagen, and other platelet agonists
• platelets fail to attach to a collagen coated surface even under low shear stress compared to control platelets that cover 53% of the surface area
• platelets fail to bind fibrinogen as measured by flow cytometry
• degranulation of mutant platelets to low levels of agonist is half that of chimeric controls
• Mn2+ addition enables platelets to bind fibrinogen
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• thrombus formation of injured mesenteric arterioles does not occur in mice containing bone marrow with the conditional knockout
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• all chimeric mice containing bone marrow with the conditional knockout bleed longer than 15 minutes compared to control chimeras that on average stop bleeding at 7.3 minutes
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