Phenotypes Associated with This Genotype

Genotype
MGI:3777753

cx12

• Allelic Composition: H2^b/H2^b; Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(TcraN15,TcrbN15)L2Elre/?
• Genetic Background: involves: 129S/SvEv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell proliferation ( J:133112 )

• CD8 T cells proliferate when presented VSV8 peptide by antigen presenting cells

arrested T cell differentiation ( J:133112 )

• the majority of T cells in the thymus are arrested at the double positive stage

• single positive CD4 positive T cells are absent in the thymus

• there is a reduced percentage of single positive CD8 positive T cells

• however, the TCR transgene rescues the arrestment of T cells in the double negative stage that occurs in Rag2 deficient background

• injection of the VSV8 antigen leads to rapid apoptosis and depletion of double-positive thymocytes with a 3-6 fold drop in total number of thymocytes

decreased CD4-positive, alpha-beta T cell number ( J:133112 )

• there are almost 10-fold less CD4 T cells in the spleen

increased CD8-positive, alpha-beta T cell number ( J:133112 )

• there are about 4-fold more CD8 T cells in the spleen

abnormal cytotoxic T cell physiology ( J:133112 )

• CD8 positive T cells exhibit increased cytotoxicity towards cells loaded with VSV8 peptide

hematopoietic system

increased T cell proliferation ( J:133112 )

• CD8 T cells proliferate when presented VSV8 peptide by antigen presenting cells

arrested T cell differentiation ( J:133112 )

• the majority of T cells in the thymus are arrested at the double positive stage

• single positive CD4 positive T cells are absent in the thymus

• there is a reduced percentage of single positive CD8 positive T cells

• however, the TCR transgene rescues the arrestment of T cells in the double negative stage that occurs in Rag2 deficient background

• injection of the VSV8 antigen leads to rapid apoptosis and depletion of double-positive thymocytes with a 3-6 fold drop in total number of thymocytes

decreased CD4-positive, alpha-beta T cell number ( J:133112 )

• there are almost 10-fold less CD4 T cells in the spleen

increased CD8-positive, alpha-beta T cell number ( J:133112 )

• there are about 4-fold more CD8 T cells in the spleen

abnormal cytotoxic T cell physiology ( J:133112 )

• CD8 positive T cells exhibit increased cytotoxicity towards cells loaded with VSV8 peptide

cellular

increased T cell proliferation ( J:133112 )

• CD8 T cells proliferate when presented VSV8 peptide by antigen presenting cells