Analysis Tools
mortality/aging
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• mice exhibit an increase in mortality compared to wild-type mice following diphtheria treatment and cecal ligation puncture (CLP) (100% compared to 45% mortality)
• however, replacement of dendritic cells with wild-type dendritic cells improves survival (mortality drops from 100% to 65%)
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immune system
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• conventional T cell, macrophages, B cell, NK cell, and NK T cell numbers are unaffected by treatment with diphtheria toxin
(J:96123)
• depletion of Cd11c+ dendritic cells by treatment with diphtheria toxin does not affect accumulation and localization of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in T cell zones of the lymph node and spleen
(J:122114)
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• 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
(J:96123)
• CD11c+ (Itgax+) dendritic cells in lung parenchyma are depleted following treatment with diphtheria toxin
(J:100867)
• treatment with diphtheria toxin depletes CD11chighMHCIIhigh dendritic cells
(J:113232)
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• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
• following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin
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• unlike in wild-type mice, NK T cell activation measured by specific cell lysis following infection with Leishmania infantum in diphtheria toxin treated mice does not occur
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• following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma and 8% for IL-4 compared to 32% and 30%, respectively, of similarly treated wild-type NK T cells
• however, normal levels of IFN-gamma and IL-4 production where observed in liver NK T cells
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• following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
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• following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
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• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
• following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma compared to 32% of similarly treated wild-type NK T cells
• however, normal levels of production where observed in liver NK T cells
• following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin
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• unlike in wild-type mice, IL-12p40 production following infection with Leishmania infantum in diphtheria toxin treated mice does not occur
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• following treatment with diphtheria toxin and stimulation with alpha-GalCer, 8% of spleen NK T cells stain positive for IL-4 compared to 30% of similarly treated wild-type NK T cells
however, normal levels of production where observed in liver NK T cells
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• mice exhibit an increase in mortality compared to wild-type mice following diphtheria treatment and cecal ligation puncture (CLP) (100% compared to 45% mortality)
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homeostasis/metabolism
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• following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
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• following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
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hematopoietic system
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• 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
(J:96123)
• CD11c+ (Itgax+) dendritic cells in lung parenchyma are depleted following treatment with diphtheria toxin
(J:100867)
• treatment with diphtheria toxin depletes CD11chighMHCIIhigh dendritic cells
(J:113232)
|
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• following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
• following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin
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• unlike in wild-type mice, NK T cell activation measured by specific cell lysis following infection with Leishmania infantum in diphtheria toxin treated mice does not occur
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• following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma and 8% for IL-4 compared to 32% and 30%, respectively, of similarly treated wild-type NK T cells
• however, normal levels of IFN-gamma and IL-4 production where observed in liver NK T cells
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