Analysis Tools
mortality/aging
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• 40% of the mice die within the first few months
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immune system
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• bone marrow cells die of apoptosis when cultured in WEHI-3 cell-conditioned media that normally supports mast cell differentiation
• mutant bone marrow cells fail to produce mast cells as wild-type bone marrow does when transferred into mast cell deficient kitw-v mice
• however, mast cells can be derived from bone marrow cells when cultured with IL-3 and SCF
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• mast cells are absent in these mice as determined by histology and flow cytometry
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• reduction of CD4+CD25+ suppressor T cells
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• bone marrow derived mast cells die of apoptosis when culture is supplemented with only low levels of IL-3 or SCF
• 80-fold more IL-3 or 50-fold more SCF is needed than controls to prevent apoptosis in cultured mast cells
• mast cells delay entering into S-phase when cultured with SCF or IL-3 taking almost twice as long as to reach half-maximal entry into S phase
• these mast cells survive and proliferate when cultured with IL-3 and SCF
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hematopoietic system
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• bone marrow cells die of apoptosis when cultured in WEHI-3 cell-conditioned media that normally supports mast cell differentiation
• mutant bone marrow cells fail to produce mast cells as wild-type bone marrow does when transferred into mast cell deficient kitw-v mice
• however, mast cells can be derived from bone marrow cells when cultured with IL-3 and SCF
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• mast cells are absent in these mice as determined by histology and flow cytometry
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• reduction of CD4+CD25+ suppressor T cells
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• bone marrow derived mast cells die of apoptosis when culture is supplemented with only low levels of IL-3 or SCF
• 80-fold more IL-3 or 50-fold more SCF is needed than controls to prevent apoptosis in cultured mast cells
• mast cells delay entering into S-phase when cultured with SCF or IL-3 taking almost twice as long as to reach half-maximal entry into S phase
• these mast cells survive and proliferate when cultured with IL-3 and SCF
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cellular
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• bone marrow cells die of apoptosis when cultured in WEHI-3 cell-conditioned media that normally supports mast cell differentiation
• mutant bone marrow cells fail to produce mast cells as wild-type bone marrow does when transferred into mast cell deficient kitw-v mice
• however, mast cells can be derived from bone marrow cells when cultured with IL-3 and SCF
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