Phenotypes Associated with This Genotype

Genotype
MGI:3834456

• Allelic Composition: Apex1^tm1Djc/Apex1^tm1Djc
• Genetic Background: involves: 129X1/SvJ * NTac:NIHS

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:50702 )

• homozygous embryos die in utero; no embryos are recovered between days 10 to 13.5

• 25% of decidua are empty or necrotic between day 10.5 and 13.5; in litters harvested from day 7.5 to 9.5, only 15.5% of embryos recovered are homozygous versus expected 25%

embryo

abnormal embryo development ( J:50702 )

• at day 6.5, embryos have unusually high percentages of pyknotic nuclei

embryonic growth retardation ( J:50702 )

• in litters examined at day 8.5 and 9.5, embryos isolated from yolk sacs display marked growth retardation

abnormal embryonic epiblast morphology ( J:50702 )

• epiblast is abnormal at day 7.5

increased embryonic epiblast cell apoptosis ( J:50702 )

• day 6.5 epiblasts have high pyknotic indices compared to controls

abnormal visceral yolk sac morphology ( J:50702 )

• yolk sac persists to day 9.5

growth/size/body

embryonic growth retardation ( J:50702 )

• in litters examined at day 8.5 and 9.5, embryos isolated from yolk sacs display marked growth retardation

cellular

increased cellular sensitivity to gamma-irradiation ( J:50702 )

• explanted blastocysts exposed to different levels of gamma-irradiation show increased sensitivity to ionizing radiation relative to exposed heterozygous blastocysts cultures

increased embryonic epiblast cell apoptosis ( J:50702 )

• day 6.5 epiblasts have high pyknotic indices compared to controls