Phenotypes Associated with This Genotype

Genotype
MGI:3844521

• Allelic Composition: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/0
• Genetic Background: NOD.FVB-1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}/Jdk

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:137009 )

• diphtheria toxin-treated mice produce a large spike of IFN-gamma and TNF-alpha before developing cachexia and dying unlike similarly treated wild-type mice

immune system

insulitis ( J:137009 )

• irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi and wild-type myeloid dendritic cells and have been treated with diphtheria toxin develop insulitis

• however, use of plasmocytoid dendritic cells instead of myeloid dendritic cells fails to restore development of insulitis

• mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells and treated with diphtheria toxin exhibit an acceleration of insulitis

• mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi and wild-type plasmocytoid dendritic cells and treated with diphtheria toxin exhibit delay in the development of insulitis

decreased dendritic cell number ( J:137009 )

• diphtheria toxin-treated mice exhibit ablation of all major dendritic cells from the spleen, pancreas, and lymph nodes unlike similarly treated wild-type mice

• diptheria toxin-treated mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells exhibit a loss of intrapancreatic plasmacytoid dendritic cells

decreased NK T cell number ( J:137009 )

• diptheria toxin-treated mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells exhibit a loss of intrapancreatic NK T cells

• however, spleen and nondraining secondary lymphoid compartments exhibit normal NK T cell numbers and the addition of plasmocytoid dendritic cells to mice restores pancreatic NK T cell numbers

decreased macrophage cell number ( J:137009 )

• diphtheria toxin-treated mice exhibit macrophage loss that is less than 5% to 8%

abnormal professional antigen presenting cell physiology ( J:137009 )

• antigen presenting cells from irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells have been treated with diphtheria toxin fail to present either peptide or whole islet cells to naive BDC2.5 T cells over a wide range of antigen concentrations unlike PBS-treated mice

• however, additional treatment with wild-type myeloid dendritic cells restores antigen presentation

increased interferon-gamma secretion ( J:137009 )

• in diphtheria toxin-treated mice

increased tumor necrosis factor secretion ( J:137009 )

• in diphtheria toxin-treated mice

decreased susceptibility to autoimmune diabetes ( J:137009 )

• irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells have been treated with diphtheria toxin do not develop diabetes unlike PBS-treated mice

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:137009 )

N • irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells have been treated with diphtheria toxin fail to develop insulitis unlike PBS-treated mice

insulitis ( J:137009 )

• irradiated wild-type mice reconstituted with bone marrow from Tg(Itgax-DTR/EGFP)57Lan that have received Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi and wild-type myeloid dendritic cells and have been treated with diphtheria toxin develop insulitis

• however, use of plasmocytoid dendritic cells instead of myeloid dendritic cells fails to restore development of insulitis

• mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells and treated with diphtheria toxin exhibit an acceleration of insulitis

• mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi and wild-type plasmocytoid dendritic cells and treated with diphtheria toxin exhibit delay in the development of insulitis

growth/size/body

cachexia ( J:137009 )

• in diphtheria toxin-treated mice

hematopoietic system

decreased dendritic cell number ( J:137009 )

• diphtheria toxin-treated mice exhibit ablation of all major dendritic cells from the spleen, pancreas, and lymph nodes unlike similarly treated wild-type mice

• diptheria toxin-treated mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells exhibit a loss of intrapancreatic plasmacytoid dendritic cells

decreased NK T cell number ( J:137009 )

• diptheria toxin-treated mice receiving Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi cells exhibit a loss of intrapancreatic NK T cells

• however, spleen and nondraining secondary lymphoid compartments exhibit normal NK T cell numbers and the addition of plasmocytoid dendritic cells to mice restores pancreatic NK T cell numbers

decreased macrophage cell number ( J:137009 )

• diphtheria toxin-treated mice exhibit macrophage loss that is less than 5% to 8%