Phenotypes Associated with This Genotype

Genotype
MGI:3851136

• Allelic Composition: Cdc20^tm1.1Pzg/Cdc20^tm1.1Pzg
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Aberrant mitoses in Cdc20^tm1.1Pzg/Cdc20^tm1.1Pzg MEFs

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:150421 )

• die around E12.5

cellular

aneuploidy ( J:150421 )

• in cultured MEFs as early as passage 3 the percentage of cells with abnormal karyotypes in increased compared to wild-type cells

abnormal mitosis ( J:150421 )

• in MEFs, in metaphase chromosomes are misaligned and in anaphase lagging chromosomes or chromosome bridges are seen

• anaphase begins prematurely

abnormal cell death ( J:150421 )

• cell growth in cultured MEFs stops with increasing passage numbers as the result of massive cell death

• cell death can be rescued by immortalizing MEFs with the expression of the SV40 large T antigen

increased embryonic tissue cell apoptosis ( J:150421 )

• very high levels of apoptotic cell death are seen in embryos

decreased cell proliferation ( J:150421 )

• MEFs grow more slowly than controls

embryo

increased embryonic tissue cell apoptosis ( J:150421 )

• very high levels of apoptotic cell death are seen in embryos

decreased embryo size ( J:150421 )

growth/size/body

decreased embryo size ( J:150421 )