Phenotypes Associated with This Genotype

Genotype
MGI:4360265

• Allelic Composition: Tg(Camk2a-HRAS*G12V)1Sva/0
• Genetic Background: involves: 129T2/SvEmsJ * C57BL/6 * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:102160 )

N • mice exhibit normal dendritic spine density, active zone area, axon terminal volume, postsynaptic density thickness, and synapse density

abnormal synaptic vesicle clustering ( J:102160 )

• more vesicles are docked at synapses than in wild-type mice

• however, the total number of vesicles is normal

abnormal CNS synaptic transmission ( J:102160 )

• following theta burst stimulation, mice exhibit increased short term potentiation compared with similarly treated wild-type mice

• high frequency theta burst stimulation produces an greater increase in short term potentiation compared to in similarly treated wild-type mice

abnormal excitatory postsynaptic potential ( J:102160 )

• at the end of tetanus, field excitatory postsynaptic potential (fEPSP) slope is larger than in wild-type mice and Syn1^tm1Sud/Syn1^tm1Sud Tg(Camk2a-HRAS1*G12V)1Sva mice

• fEPSP amplitude is increased in response to each theta burst compared with wild-type mice

enhanced long-term potentiation ( J:102160 )

• following theta burst stimulation, mice exhibit increased long term potentiation compared with similarly treated wild-type mice with a cumulative probability distribution shift towards larger potentiation

• high frequency theta burst stimulation produces an greater increase in long term potentiation compared to in similarly treated wild-type mice

• however, pairing-induced associative long term potentiation is normal

abnormal miniature excitatory postsynaptic currents ( J:102160 )

• mice exhibit increased in frequency, but not amplitude, of miniature excitatory postsynaptic currents (mEPSCs) compared with wild-type mice

• however, treatment with the MEK inhibitor U0126 abolishes increased mEPSC frequency

enhanced paired-pulse facilitation ( J:102160 )

• mice exhibit frequency-dependent enhancement of paired-pulse facilitation compared with wild-type mice

behavior/neurological

behavior/neurological phenotype ( J:102160 )

N • mice exhibit normal long-term stability of spatial memory

enhanced contextual conditioning behavior ( J:102160 )

• mice exhibit enhanced hippocampus-dependent contextual learning compared with wild-type mice

• mice exhibit increased freezing at 2 and 24 hours after training compared with wild-type mice indicating enhanced short-term memory

• after immediate shck treatment, preexposure mice exhibit enhanced freezing unlike in similarly treated wild-type mic

• however, mice that are not preexposed exhibit normal freezing following immediate shock training

abnormal spatial learning ( J:102160 )

• in a Morris water maze, mice require less training time to learn a spatial location compared with wild-type mice