Analysis Tools
growth/size/body
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• males exhibit a slight increase in crown-rump length compared to controls at 10 weeks
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skeleton
| N |
• bone resorption is slightly decreased in both mutants and controls while osteoclast numbers are not affected
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• calvarial osteoblasts exhibit diminished differentiation potential in terms of bone nodule formation and alkaline phosphatase expression
• with prednisolone treatment, osteoblast differentiation is impaired in wild-type mice, but is unaffected in mutants, as determined by Col1a1 expression, marking functional osteoblasts
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• in response to two weeks of treatment with the Nr3c1 agonist prednisolone, mutants do not display reduced bone mineral density in vertebral bones in contrast to treated controls
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• mice show modest but highly significant decrease in bone density in calcified vertebral sections compared to controls at 3 months
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• trabecular numbers are decreased but trabecular thickness is unaltered resulting in increased trabecular spacing
• osteoblast numbers, osteoblast surface, osteocytes, and osteoclast parameters are not significantly changed compared to controls
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• bone formation in vertebrae is not inhibited whereas treated controls show near complete inhibition of bone formation with prednisolone treatment; osteoblastogenesis (numbers of colony forming units of osteoblasts) is not impaired as seen in controls with prednisolone treatment
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cellular
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• calvarial osteoblasts exhibit diminished differentiation potential in terms of bone nodule formation and alkaline phosphatase expression
• with prednisolone treatment, osteoblast differentiation is impaired in wild-type mice, but is unaffected in mutants, as determined by Col1a1 expression, marking functional osteoblasts
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