Phenotypes Associated with This Genotype

Genotype
MGI:4847917

• Allelic Composition: Stat5a^tm2Mam/Stat5a^tm2Mam; Stat5b^tm1Mam/Stat5b^tm1Mam; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

increased apoptosis ( J:155489 )

• increased cell death in GMPs and their progeny during culture with GM-CSF up to the fifth generation

abnormal neutrophil differentiation ( J:155489 )

• no elevation of neutrophils 18 days after induced myelosuppression by 5-FU injection

• severely impaired GM-CSF-induced increase of peripheral blood neutrophils

decreased cell proliferation ( J:155489 )

• longer generation times in every generation (F1 through F4) during culture with GM-CSF compared with control cells

hematopoietic system

abnormal leukopoiesis ( J:155489 )

• decreased absolute number of bone marrow mononuclear cells (BMMNCs) from both femora and tibiae per mouse

• decreased absolute number of granulocyte-macrophage progenitors (GMPs) from both femora and tibiae per mouse

• impaired colony formation of GMPs

• reduced numbers of mutant colonies by more than 50% in the presence of GM-CSF

• the average cell number of a mutant GMP-derived colony is only 11% of that from control GMPs

• normal percentage of GMPs in BMMNCs

abnormal neutrophil differentiation ( J:155489 )

• no elevation of neutrophils 18 days after induced myelosuppression by 5-FU injection

• severely impaired GM-CSF-induced increase of peripheral blood neutrophils

anemia ( J:155489 )

decreased erythrocyte cell number ( J:155489 )

decreased hematocrit ( J:155489 )

decreased hemoglobin content ( J:155489 )

decreased leukocyte cell number ( J:155489 )

• decreased white blood cell counts

decreased neutrophil cell number ( J:155489 )

• decreased absolute number of peripheral neutrophils by 50% in homeostasis 3 weeks after PolyIC injection

decreased lymphocyte cell number ( J:155489 )

• decreased lymphocytes counts (70% decrease)

decreased monocyte cell number ( J:155489 )

• decreased monocytes counts (44% decrease)

abnormal neutrophil physiology ( J:155489 )

• only 65% of mutant bone marrow neutrophils, compare with virtually all cells from control animals survive for 36 hours in the presence of GM-CSF

immune system

abnormal leukopoiesis ( J:155489 )

• decreased absolute number of bone marrow mononuclear cells (BMMNCs) from both femora and tibiae per mouse

• decreased absolute number of granulocyte-macrophage progenitors (GMPs) from both femora and tibiae per mouse

• impaired colony formation of GMPs

• reduced numbers of mutant colonies by more than 50% in the presence of GM-CSF

• the average cell number of a mutant GMP-derived colony is only 11% of that from control GMPs

• normal percentage of GMPs in BMMNCs

abnormal neutrophil differentiation ( J:155489 )

• no elevation of neutrophils 18 days after induced myelosuppression by 5-FU injection

• severely impaired GM-CSF-induced increase of peripheral blood neutrophils

decreased leukocyte cell number ( J:155489 )

• decreased white blood cell counts

decreased neutrophil cell number ( J:155489 )

• decreased absolute number of peripheral neutrophils by 50% in homeostasis 3 weeks after PolyIC injection

decreased lymphocyte cell number ( J:155489 )

• decreased lymphocytes counts (70% decrease)

decreased monocyte cell number ( J:155489 )

• decreased monocytes counts (44% decrease)

abnormal neutrophil physiology ( J:155489 )

• only 65% of mutant bone marrow neutrophils, compare with virtually all cells from control animals survive for 36 hours in the presence of GM-CSF