Analysis Tools
mortality/aging
|
• only 10% of mice survived to 6 weeks of age
• 90% of mice were euthanized at 4-5 weeks of age due to end stage renal failure and nephrotic syndrome
|
behavior/neurological
|
• mice become less physically active at 3 weeks of age
|
growth/size/body
|
• at 6 weeks of age, mice are smaller than control littermates
|
homeostasis/metabolism
|
• mice develop severe proteinuria by 6 weeks of age
|
albuminuria
(
J:135414
)
|
• albuminuria is already evident at P1
• marked albuminuria is noted at 6 weeks of age
|
renal/urinary system
|
• a significant increase in podocyte apoptosis is detected at P10 and P21 by TUNEL analysis
• at P21, mice exhibit about one-third of the podocyte number found in control littermates, as quantified in EM sections
|
|
• mice develop severe proteinuria by 6 weeks of age
|
albuminuria
(
J:135414
)
|
• albuminuria is already evident at P1
• marked albuminuria is noted at 6 weeks of age
|
|
• mice develop end stage kidney disease with pathological changes in the glomeruli and tubulo-interstitium
|
|
• at 6 weeks of age, many Bowmans capsules are either empty or contain partially degenerated glomeruli
|
|
• foot process effacement is first noted at E15.5
• extensive foot process effacement is noted at P10 and progresses by P21
|
|
• early segmental splitting of the glomerular basement membrane (GBM) is first noted at P10 and progresses by P21
• however, normal GBM morphogenesis is noted at E15.5 and at P1
|
|
• at P21, mice exhibit degeneration of the capillary loops and mesangium with little glomerulosclerosis
• at 6 weeks of age, mice exhibit dilated glomerular capillaries and glomerular disintegration
|
|
• by P21, mice exhibit degeneration of the capillary loops
• however, normal glomerular capillary morphogenesis is noted at E15.5 and at P1
|
|
• at P10, some glomeruli display segmentally "ballooned" capillary lumens; more "ballooned" capillary loops are noted at 3 weeks of age
• at 6 weeks of age, mice exhibit dilated glomerular capillaries
|
|
• mesangium hypercellularity is observed by 3 weeks of age
|
|
• increased mesangial matrix with focal defects is noted at 3 weeks, indicating mesangial injury
|
|
• multiple cytoplasmic vacuoles are first noted in mesangial cells at P10
• by P21, mice exhibit degeneration of the mesangium with little glomerulosclerosis
|
|
• at P10, mice exhibit multiple cytoplasmic vacuoles within the tubular epithelial cells
• flattened epithelial cells with extensive proteinaceous tubular casts are noted at 6 weeks of age
|
small kidney
(
J:135414
)
|
• at 6 weeks of age, end stage kidneys are smaller than those of age-matched control mice
|
|
• dilated renal tubules containing hyaline material are observed at 6 weeks of age
• tubular dilatation is first evident at P10 and increased by 3 weeks of age
|
renal cast
(
J:135414
)
|
• extensive proteinaceous tubular casts are noted at 6 weeks of age
|
pale kidney
(
J:135414
)
|
• at 6 weeks of age, end stage kidneys are paler than those of age-matched control mice
|
cardiovascular system
|
• by P21, mice exhibit degeneration of the capillary loops
• however, normal glomerular capillary morphogenesis is noted at E15.5 and at P1
|
|
• at P10, some glomeruli display segmentally "ballooned" capillary lumens; more "ballooned" capillary loops are noted at 3 weeks of age
• at 6 weeks of age, mice exhibit dilated glomerular capillaries
|
cellular
|
• mesangium hypercellularity is observed by 3 weeks of age
|
|
• a significant increase in podocyte apoptosis is detected at P10 and P21 by TUNEL analysis
• at P21, mice exhibit about one-third of the podocyte number found in control littermates, as quantified in EM sections
|
