Phenotypes Associated with This Genotype

Genotype
MGI:5520923

• Allelic Composition: Pla2g2d^tm1.1Mhg/Pla2g2d^tm1.1Mhg
• Genetic Background: B6NCrSlc.Cg-Pla2g2d^tm1.1Mhg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:201008 )

N • mice exhibit normal dendritic cell, T cell, B cell and macrophage populations in spleen, inguinal and mesenteric lymph nodes, Peyer's patch and thymus

increased dendritic cell number ( J:201008 )

• of activated dendritic cells in hapten-treated mice

abnormal effector T cell morphology ( J:201008 )

• increased in hapten-treated mice

increased CD4-positive, alpha-beta T cell number ( J:201008 , J:229035 )

• slight increase in the proportion of splenic CD4+ T cells (J:201008)

• lungs of SARS-CoV-infected mice exhibit a higher frequency and number of virus-specific CD4 T cells (J:229035)

increased CD8-positive, alpha-beta T cell number ( J:229035 )

• lungs of SARS-CoV-infected mice exhibit a higher frequency and number of virus-specific CD8 T cells

abnormal cytotoxic T cell physiology ( J:229035 )

• SARS-CoV-infected mice show augmented CD8 T cell cytolytic activity

• CD8 T cell responses are higher in the lungs of influenza A virus-infected mice at 10 days post infection

abnormal dendritic cell physiology ( J:201008 )

• hyperactive following LPS treatment

abnormal dendritic cell migration ( J:229035 )

• mice exhibit a higher frequency and number of respiratory dendritic cells (rDCs) that migrate to the mediastinal lymph nodes (MLN) than wild-type mice

• SARS-CoV-infected middle-aged mice show a higher frequency and number of rDCs that migrate from lungs to MLN compared to infected wild-type mice

• influenza A virus-infected mice show a higher frequency and number of rDCs in the MLN at 18 hours post infection

increased susceptibility to type IV hypersensitivity reaction ( J:201008 )

• hapten-treated mice exhibit impaired resolution (determined by ear swelling, increased activated dendritic cells and increased effector T cells) compared with wild-type mice

• however, mice exhibit normal antigen uptake, recruitment and activation of skin dendritic cells migrating into the lymph nodes

increased interferon-gamma secretion ( J:229035 )

• CD8 T cell from SARS-CoV-infected mice produce more IFN-gamma per cell

decreased interleukin-6 secretion ( J:201008 )

• from bone marrow-derived dendritic cells in response to suboptimal LPS treatment

decreased tumor necrosis factor secretion ( J:201008 )

• from bone marrow-derived dendritic cells in response to suboptimal LPS treatment

decreased susceptibility to Coronaviridae infection ( J:229035 )

• middle-aged mice show decreased susceptibility to infection with SARS-CoV MA15 , showing increased survival, increased viral clearance mediated by a more robust T cell response, and decreased pathological changes in the lungs

decreased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:229035 )

• more than 80% of middle-aged mice infected with SARS-CoV MA15 survive the infection compared to 0% survival in controls

• however, mice infected with the PR8 strain of influenza A virus show similar survival as controls

mortality/aging

decreased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:229035 )

• more than 80% of middle-aged mice infected with SARS-CoV MA15 survive the infection compared to 0% survival in controls

• however, mice infected with the PR8 strain of influenza A virus show similar survival as controls

homeostasis/metabolism

abnormal lipid homeostasis ( J:201008 )

• decreased arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid in lymph nodes

decreased unsaturated fatty acids level ( J:229035 )

• middle-aged, but not young, mice show lower levels of arachidonic acid

• middle-aged mice infected with SARS-CoV exhibit lower levels of free polyunsaturated fatty acids, arachidonic acid, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) in the lungs than controls

hematopoietic system

abnormal dendritic cell migration ( J:229035 )

• mice exhibit a higher frequency and number of respiratory dendritic cells (rDCs) that migrate to the mediastinal lymph nodes (MLN) than wild-type mice

• SARS-CoV-infected middle-aged mice show a higher frequency and number of rDCs that migrate from lungs to MLN compared to infected wild-type mice

• influenza A virus-infected mice show a higher frequency and number of rDCs in the MLN at 18 hours post infection

increased dendritic cell number ( J:201008 )

• of activated dendritic cells in hapten-treated mice

abnormal effector T cell morphology ( J:201008 )

• increased in hapten-treated mice

increased CD4-positive, alpha-beta T cell number ( J:201008 , J:229035 )

• slight increase in the proportion of splenic CD4+ T cells (J:201008)

• lungs of SARS-CoV-infected mice exhibit a higher frequency and number of virus-specific CD4 T cells (J:229035)

increased CD8-positive, alpha-beta T cell number ( J:229035 )

• lungs of SARS-CoV-infected mice exhibit a higher frequency and number of virus-specific CD8 T cells

abnormal cytotoxic T cell physiology ( J:229035 )

• SARS-CoV-infected mice show augmented CD8 T cell cytolytic activity

• CD8 T cell responses are higher in the lungs of influenza A virus-infected mice at 10 days post infection

cellular

abnormal dendritic cell migration ( J:229035 )

• mice exhibit a higher frequency and number of respiratory dendritic cells (rDCs) that migrate to the mediastinal lymph nodes (MLN) than wild-type mice

• SARS-CoV-infected middle-aged mice show a higher frequency and number of rDCs that migrate from lungs to MLN compared to infected wild-type mice

• influenza A virus-infected mice show a higher frequency and number of rDCs in the MLN at 18 hours post infection