Analysis Tools
reproductive system
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• significantly increased number of TUNEL+ apoptotic cells per seminiferous tubule at 8, 14 and 28 weeks of age relative to wild-type controls
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• significantly decreased seminal vesicle + coagulating gland weight at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
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• at 8 weeks of age, all seminiferous tubules display a basal single layer of spermatogonia and contain Sertoli cells along with 2-5 layers of primary spermatocytes
• no mature spermatozoa are found in the lumen of seminiferous tubules at 8 weeks
• cells suspected as being round spermatids are found near the central lumen at 14 and 28 weeks of age
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• significantly decreased testis weight at 8, 14 and 28 weeks of age relative to wild-type controls
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• disruption of spermatogenesis before the first meiotic division, consistent with presence of pachytene spermatocytes at 8 weeks of age, as revealed by gamma-H2AX labeling
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azoospermia
(
J:232496
)
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• complete absence of epididymal sperm
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• presumed round spermatids are detected only in ~25% of seminiferous tubules at 14 and 28 weeks of age
• round spermatids appear abnormal, with acrosin accumulating throughout the cytoplasm instead of forming a cap-like structure on the nuclear envelope as in wild-type round spermatids
• number of round spermatids per tubule cross-section is reduced relative to wild-type controls
• however, no evidence of any elongating or elongated spermatids is observed
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• at 8 weeks of age, some spermatocytes appear to undergo nuclear condensation and pyknosis
• eosinophilic staining of spermatocyte cytoplasm indicates progressive degeneration and apoptosis
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• meiotic arrest at the spermatocyte stage at 8 weeks of age
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• male homozygotes display delayed puberty, as shown by normal testosterone levels and accessory gland weights at 14 and 28 weeks of age
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• continuous mating of male homozygotes with fertile females does not result in pregnancy
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endocrine/exocrine glands
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• significantly decreased seminal vesicle + coagulating gland weight at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
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• at 8 weeks of age, all seminiferous tubules display a basal single layer of spermatogonia and contain Sertoli cells along with 2-5 layers of primary spermatocytes
• no mature spermatozoa are found in the lumen of seminiferous tubules at 8 weeks
• cells suspected as being round spermatids are found near the central lumen at 14 and 28 weeks of age
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• significantly decreased testis weight at 8, 14 and 28 weeks of age relative to wild-type controls
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homeostasis/metabolism
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• significantly decreased plasma testosterone levels at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
• however, no changes in Leydig cell number and/or size are detected between 8 and 28 weeks of age
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cellular
azoospermia
(
J:232496
)
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• complete absence of epididymal sperm
|
|
|
• presumed round spermatids are detected only in ~25% of seminiferous tubules at 14 and 28 weeks of age
• round spermatids appear abnormal, with acrosin accumulating throughout the cytoplasm instead of forming a cap-like structure on the nuclear envelope as in wild-type round spermatids
• number of round spermatids per tubule cross-section is reduced relative to wild-type controls
• however, no evidence of any elongating or elongated spermatids is observed
|
|
|
• at 8 weeks of age, some spermatocytes appear to undergo nuclear condensation and pyknosis
• eosinophilic staining of spermatocyte cytoplasm indicates progressive degeneration and apoptosis
|
|
|
• meiotic arrest at the spermatocyte stage at 8 weeks of age
|
|
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• significantly increased number of TUNEL+ apoptotic cells per seminiferous tubule at 8, 14 and 28 weeks of age relative to wild-type controls
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