Phenotypes Associated with This Genotype

Genotype
MGI:6263201

• Allelic Composition: Polr1a^{tm1b(EUCOMM)Hmgu}/Polr1a^{tm1b(EUCOMM)Hmgu}
• Genetic Background: C57BL/6N-Polr1a^{tm1b(EUCOMM)Hmgu}/Bay
• Cell Lines: HEPD0779_7_B03

Data Sources

IMPC Data for Polr1a

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Polr1a^{tm1b(EUCOMM)Hmgu}/Polr1a^{tm1b(EUCOMM)Hmgu} mice exhibit embryonic lethality, with only 3% of embryos recovered at E3.5 as dying 2-cell stage embryos, suggesting zygotic genome activation failure.

embryo

abnormal preimplantation embryo development ( J:279207 )

• only dying 2-cell stage embryos are observed at E3.5, suggesting failure of zygotic genome activation

failure of blastocyst formation ( J:279207 )

• 2-cell stage embryos fail to reach a morphological blastocyst stage after 3 days in culture

embryonic growth arrest ( J:279207 )

• 2-cell stage embryos recovered at E3.5 appear to be dying

• in vitro, embryos show no further development after 3 days in culture

mortality/aging

preweaning lethality, complete penetrance ( J:211773 )

IMPC - BCM

embryonic lethality before implantation, complete penetrance ( J:279207 )

• only a few homozygous embryos are recovered at E3.5 (3% versus expected 25%, n=36) and appear as dying 2-cell stage embryos; no embryos or excess resorption sites are observed at E7.5