Phenotypes Associated with This Genotype

Genotype
MGI:6356967

• Allelic Composition: Plek2^tm1Pji/Plek2^tm1Pji; Jak2^tm1.1Ble/Jak2⁺; Commd10^{Tg(Vav1-icre)A2Kio}/Commd10⁺
• Genetic Background: involves: 129 * C57BL/6 * C57BL/10 * CBA/Ca

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:257910 )

• more than 80% of mice survive beyond 40 weeks

• mice treated with phenylhydrazine to further reduce red blood counts show an even greater improvement in survival

hematopoietic system

hematopoietic system phenotype ( J:257910 )

N • mice exhibit amelioration of the neutrophilia and thrombocytosis, partial reversion of reticulocytosis, mild reduction in red blood cell counts, fewer megakaryocytes and megakaryocyte clusters, reduced spleen size, and reversion of about 25% of the red blood cell expansion seen in single Jak2^tm1.1Ble conditional mutants

N • bone marrow lineage-negative hematopoietic stem and progenitor cells grown in culture show a reduction in the increased proliferation of erythroid and granulocytic cells seen in single Jak2^tm1.1Ble conditional mutants

abnormal granulocyte differentiation ( J:257910 )

• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells

abnormal megakaryocyte differentiation ( J:257910 )

• early-stage megakaryocytic differentiation to CD41+ cells is mildly decreased in cultured cells

extramedullary hematopoiesis ( J:257910 )

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:257910 )

N • mice show decreased vascular occlusions than in single Jak2^tm1.1Ble conditional mutants, with a decrease in the number of large clots with compact red blood cells and improved blood flow

immune system

immune system phenotype ( J:257910 )

N • several proinflammatory cytokines, including CCL3 and CSF1, that are increased in single Jak2^tm1.1Ble conditional mutants are reduced

abnormal granulocyte differentiation ( J:257910 )

• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells

cellular

abnormal cell differentiation ( J:257910 )

• erythroid differentiation to Ter119/CD71 double-positive cells is mildly decreased in cultured cells

abnormal granulocyte differentiation ( J:257910 )

• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells

abnormal megakaryocyte differentiation ( J:257910 )

• early-stage megakaryocytic differentiation to CD41+ cells is mildly decreased in cultured cells