Phenotypes Associated with This Genotype

Genotype
MGI:6400930

• Allelic Composition: Tafa1^{tm1(KOMP)Vlcg}/Tafa1^{tm1(KOMP)Vlcg}
• Genetic Background: C57BL/6N-Tafa1^{tm1(KOMP)Vlcg}
• Cell Lines: 16914A-F3

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:284736 )

N • males show normal % of freezing in training, cue tests, and context tests during trace fear conditioning tests, and normal time of latency during the hot plate test

N • females show no significant differences in food intake, meal number or meal size relative to wild-type females

N • despite locomotor hyperactivity, both sexes show normal short-term spatial memory and exploratory behavior in the Y-maze spontaneous alternation task

increased eating frequency ( J:284736 )

♂ • in males, increased food intake in the light cycle is due to increased meal frequency (not meal size)

decreased food intake ( J:284736 )

♂ • males show significantly decreased food intake in the dark cycle relative to wild-type males

increased food intake ( J:284736 )

♂ • males show significantly increased food intake in the light cycle relative to wild-type males

♂ • however, total food intake over a 24-h period is normal in males

decreased anxiety-related response ( J:284736 )

♀ • in elevated plus maze (EPM) tests, females show a significant increase in the % of time spent in open arms and in distance traveled relative to wild-type females

decreased freezing behavior ( J:284736 )

♀ • females show decreased freezing in response to the first (before any fear conditioning occurs) and subsequent electric foot shocks during the training period, impeding an unambiguous interpretation of reduced fear conditioning

increased locomotor activity ( J:284736 )

• males show significantly increased total physical and ambulatory activity in the light cycle and significantly higher ambulatory activity in the dark cycle relative to wild-type males

• females show strikingly increased total physical and ambulatory activity over a 24- h period relative to wild-type females; effect is more pronounced in the light cycle and trends higher in the dark cycle

• in an open field test, both males and females show significantly increased total and central locomotor activity over a 30-min period relative to control mice

• degree of locomotor hyperactivity is more pronounced in females than male mice

increased thermal nociceptive threshold ( J:284736 )

♀ • females show significantly longer times of latency in the hot plate test, suggesting reduced pain in response to heat

♀ • however, females show a normal scratching response in the behavioral itch assay

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:284736 )

♂N • males show no significant differences in metabolic rate (VO₂), substrate oxidation (respiratory exchange ratio, RER), or energy expenditure (EE) relative to wild-type males

abnormal nervous system dopamine level ( J:284736 )

♀ • females show significantly increased dopamine turnover (i.e. ratio of dopamine metabolites to dopamine) in the striatum relative to wild-type females

♀ • however, serotonin turnover rate (ratio of serotonin metabolite 5-HIAA to 5-HT) is normal

increased noradrenaline level ( J:284736 )

♀ • females show significantly increased norepinephrine, but not dopamine, levels in the striatum relative to wild-type females

increased energy expenditure ( J:284736 )

♀ • females show a significant increase in energy expenditure relative to wild-type females

increased basal metabolism ( J:284736 )

♀ • females show a significant increase in metabolic rate (VO₂) relative to wild-type females

nervous system

nervous system phenotype ( J:284736 )

N • both sexes exhibit normal sensorimotor gating as measured by PPI of the acoustic startle response test

growth/size/body

growth/size/body region phenotype ( J:284736 )

♂N • males show no significant difference in whole-body fat and lean mass relative to wild-type males