Phenotypes for Ebi3 MGI:6442003 MGI Mouse

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:286951 )

• mice infected intracranially with mouse hepatitis virus strain J2.2v-1 (JHMV) show increased susceptibility to JHMV-induced death, with approximately 70% dying by 12 days post infection (dpi) compared to about 30% of wild-type mice

abnormal CD4-positive, alpha-beta T cell number ( J:286951 )

• JHMV-infected mice show reduced numbers of CD4+ T cell within the brain at 5 dpi but levels return to normal at 7 and 12 dpi

• JHMV-infected mice show reduced numbers of virus-specific CD4+ T cells within the CNS at 7 dpi but increased numbers by 12 dpi

• JHMV-infected mice show decreased numbers of virus-specific CD4+ T cells isolated from spleens

increased CD8-positive, alpha-beta T cell number ( J:286951 )

• JHMV-infected mice show reduced numbers of CD8+ T cells within the brains at 5 dpi but numbers are increased by 7 dpi and this increase in sustained to 12 dpi

• JHMV-infected mice show increased numbers of virus-specific CD8+ T cells within the CNS at 7 and 12 dpi

• however, numbers of virus-specific CD8+ T cells present in the spleen are similar to wild-type

increased macrophage cell number ( J:286951 )

• macrophage infiltration is elevated in JHMV-infected mice at 7 and 12 dpi

abnormal T cell activation ( J:286951 )

• CD4+ and CD8+ T cells show enhanced activation (CD62L-CD25+) states within the central nervous system (CNS)

increased interferon-gamma secretion ( J:286951 )

• JHMV virus-specific CD4+ T cells exhibit an approximate 2-fold increase in IFN-gamma production and CD8+ T cells show a more than 2.5-fold increase in IFN-gamma levels in response to peptide stimulation compared to wild-type mice

decreased interleukin-10 secretion ( J:286951 )

• JHMV virus-specific T cells show a reduction in 1L-10 production

brain inflammation ( J:286951 )

• JHMV-infected mice show increased thickening of the meninges and increased perivascular cuffing indicating enhanced neuroinflammation

increased susceptibility to Coronaviridae infection ( J:286951 )

• mice exhibit increased sensitivity to mouse hepatitis virus strain J2.2v-1 (JHMV) infection, showing elevated titers by 7 days post infection (dpi) which are reduced to below detection by 12 dpi indicating ability to control viral replication but showing increased disease severity with enhanced leukocyte infiltration and T cell activation and increased IFN-gamma secretion