Phenotypes Associated with This Genotype

Genotype
MGI:6725111

• Allelic Composition: Tfb2m^{tm1a(KOMP)Wtsi}/Tfb2m⁺; Tg(Ins2-cre)23Herr/0
• Genetic Background: involves: C57BL/6J * C57BL/6N * CBA/J
• Cell Lines: EPD0042_2_F02

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased insulin secretion ( J:256448 )

♂ • impaired glucose-stimulated as well as alpha-KIC- and KCl-stimulated insulin secretion in pancreatic islets isolated from 7-month old mice

♂ • decreased pancreatic insulin content per weight of pancreas at 7 months of age

♂ • however, islet insulin content is unchanged

decreased circulating insulin level ( J:256448 )

♂ • reduced plasma insulin level in non-fasted mice at 7 months of age

impaired glucose tolerance ( J:256448 )

♂ • increased total area under the curve (AUC) for plasma glucose (with a tendency toward impaired acute insulin response) during i.v. glucose tolerance testing in mice at 6 months of age

endocrine/exocrine glands

decreased pancreatic beta cell mass ( J:256448 )

♂

decreased pancreatic islet number ( J:256448 )

♂

decreased insulin secretion ( J:256448 )

♂ • impaired glucose-stimulated as well as alpha-KIC- and KCl-stimulated insulin secretion in pancreatic islets isolated from 7-month old mice

♂ • decreased pancreatic insulin content per weight of pancreas at 7 months of age

♂ • however, islet insulin content is unchanged

cellular

abnormal mitochondrial physiology ( J:256448 )

♂ • after stimulation with 16.7 mM glucose, maximal decrease in TMRM fluorescence intensity is reduced by 64% in pancreatic islets isolated from 7-month old mice

abnormal mitochondrial ATP synthesis coupled electron transport ( J:256448 )

♂ • increased basal levels of ATP in pancreatic islets isolated from 7-month old mice

♂ • however, upon stimulation with 22 mM glucose, islet ATP content is lower than that in control islets