Analysis Tools
reproductive system
 N |
• females exhibit normal ovarian histology and follicle counts at 18 days (prepubertal) and at 7 months of age, indicating normal folliculogenesis
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• reduced female fertility is likely due to subtle defects in oogenesis resulting in smaller oocytes with compromised cytoplasmic competence
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• fully grown oocytes from hyperstimulated females are significantly smaller than control oocytes, as determined by germinal vesicle diameter
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• in vitro oocyte maturation (IVM) analysis revealed that only 80% of germinal vesicle-intact oocytes arrested in prophase of meiosis I are able to resume meiosis and reach metaphase of meiosis I (MI) within 8 h relative to ~90% in controls
• however, spindle morphology and chromosome alignment are normal at the MI stage and no chromosome segregation defects are observed following IVM
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• females show a mild subfertility phenotype
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• when bred with wild type male mice for 6 months, adult females produce a significantly lower average litter size than controls
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cellular
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• reduced female fertility is likely due to subtle defects in oogenesis resulting in smaller oocytes with compromised cytoplasmic competence
|
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• fully grown oocytes from hyperstimulated females are significantly smaller than control oocytes, as determined by germinal vesicle diameter
|
|
|
• in vitro oocyte maturation (IVM) analysis revealed that only 80% of germinal vesicle-intact oocytes arrested in prophase of meiosis I are able to resume meiosis and reach metaphase of meiosis I (MI) within 8 h relative to ~90% in controls
• however, spindle morphology and chromosome alignment are normal at the MI stage and no chromosome segregation defects are observed following IVM
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embryo
| N |
• in culture, the % of zygotes progressing to the blastocyst stage is not significantly different from that in controls, suggesting normal preimplantation embryo development
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