Resttm1a(EUCOMM)Wtsi
Targeted Allele Detail
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| Symbol: |
Resttm1a(EUCOMM)Wtsi |
| Name: |
RE1-silencing transcription factor; targeted mutation 1a, Wellcome Trust Sanger Institute |
| MGI ID: |
MGI:4433924 |
| Gene: |
Rest Location: Chr5:77413338-77434279 bp, + strand Genetic Position: Chr5, 41.61 cM
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| Alliance: |
Resttm1a(EUCOMM)Wtsi page
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| IMPC: |
Rest gene page |
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| Mutant Cell Lines: |
EPD0105_1_C06, EPD0105_1_E05 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:224858
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| Parent Cell Line: |
JM8.F6 (ES Cell)
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| Strain of Origin: |
C57BL/6N
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| Project Collection: |
EUCOMM
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| Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
| Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 77415210 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 77416960. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:155845, J:173534, J:224858)
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Cell line EPD0105_1_E05 was successfully used to make chimeric mice. Germline transmission was accomplished. J:224858 |
| Original: |
J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010; |
| All: |
3 reference(s) |
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Analysis Tools
