Moktm1a(EUCOMM)Hmgu
Targeted Allele Detail
|
|
| Symbol: |
Moktm1a(EUCOMM)Hmgu |
| Name: |
MOK protein kinase; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH |
| MGI ID: |
MGI:4841489 |
| Gene: |
Mok Location: Chr12:110774232-110807373 bp, - strand Genetic Position: Chr12, 60.81 cM
|
| Alliance: |
Moktm1a(EUCOMM)Hmgu page
|
| IMPC: |
Mok gene page |
|
| Mutant Cell Lines: |
HEPD0530_7_A09, HEPD0530_7_A11, HEPD0530_7_A12, HEPD0530_7_B10, HEPD0530_7_B11, HEPD0530_7_C09, HEPD0530_7_C12, HEPD0530_7_D12, HEPD0530_7_E09, HEPD0530_7_E12, HEPD0530_7_F09, HEPD0530_7_F10, HEPD0530_7_H11 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:204812
|
| Parent Cell Line: |
JM8.N4 (ES Cell)
|
| Strain of Origin: |
C57BL/6N
|
| Project Collection: |
EUCOMM
|
|
| Allele Type: |
|
Targeted (Conditional ready, Null/knockout, Reporter) |
| Mutation: |
|
Insertion
Vector: L1L2_Pgk_P
|
| |
|
Mutation details: The L1L2_Pgk_P cassette was inserted at position 110781160 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 110782130. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
|
|
|
|
|
| Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
| All: |
3 reference(s) |
|
Analysis Tools
