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Gene Expression Data
Assay Details
Assay
Reference: J:39086 Marcos MA, et al., Antigenic phenotype and gene expression pattern of lymphohemopoietic progenitors during early mouse ontogeny. J Immunol. 1997 Mar 15;158(6):2627-37
Assay type: RT-PCR
MGI Accession ID: MGI:2670384
Gene symbol: Rag1
Gene name: recombination activating 1
Probe: F3, R6
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The RT-PCR reaction was electrophoresed, Southern blotted and hybridized with a probe (MGI:2670373).
Results Image: 4A RAG-1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
9 E9.5 (a) TS15: dorsal aorta
TS15: gut
TS15: mesenchyme derived from splanchnopleure
TS15: vitelline artery 		Not Specified 0.1-1 µg; total RNA BALB/c Not Specified The tissue is P-Sp or Para-aortic splanchnopleura which includes: paired dorsal aorta, developing gut, omphalomesenteric (vitelline) artery and the splanchnic mesoderm.
9.5 E10.0 (a) TS16: dorsal aorta
TS16: gut
TS16: mesenchyme derived from splanchnopleure
TS16: vitelline artery 		Present (b) 0.1-1 µg; total RNA BALB/c Not Specified The tissue is P-Sp or Para-aortic splanchnopleura which includes: paired dorsal aorta, developing gut, omphalomesenteric (vitelline) artery and the splanchnic mesoderm.
10.5 E11.0 (a) TS18: aorta-gonad-mesonephros
TS18: gut 		Present 0.1-1 µg; total RNA BALB/c Not Specified The tissue is AGM or aorta (arterial system), gonads, mesonephros, but the authors state that at the ages examined also included mesentery and gut.
11 E11.5 (a) TS19: aorta-gonad-mesonephros
TS19: gut 		Weak (c) 0.1-1 µg; total RNA BALB/c Not Specified The tissue is AGM or aorta (arterial system), gonads, mesonephros, but the authors state that at the ages examined also included mesentery and gut.
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression at this time point was present in 4 of 6 samples.
(c) Expression was down-regulated at E11.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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