Rag1 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:39086 Marcos MA, et al., Antigenic phenotype and gene expression pattern of lymphohemopoietic progenitors during early mouse ontogeny. J Immunol. 1997 Mar 15;158(6):2627-37
Assay type:	RT-PCR
MGI Accession ID:	MGI:2670388
Gene symbol:	Rag1
Gene name:	recombination activating 1

Probe:	F3, R6
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	The RT-PCR reaction was electrophoresed, Southern blotted and hybridized with a probe (MGI:2670373).

Results

Image: 4B RAG-1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
8	E8.5 (a)	TS13: embryo ectoderm TS13: head TS13: musculature	Absent	0.1-1 µg; total RNA	BALB/c		Not Specified	remainder of embryo after dissection of the P-Sp (para-aortic splanchnopleure-the paired dorsal aorta, gut, omphalomesenteric artery, and the splanchnic mesoderm), yolk sac and liver.
9	E9.5 (a)	TS15: appendage TS15: embryo ectoderm TS15: embryo mesoderm TS15: head TS15: musculature TS15: musculoskeletal system TS15: respiratory system	Absent	0.1-1 µg; total RNA	BALB/c		Not Specified	remainder of embryo after dissection of the P-Sp (para-aortic splanchnopleure-the paired dorsal aorta, gut, omphalomesenteric artery, and the splanchnic mesoderm), yolk sac and liver.
9.5	E10.0 (a)	TS16: appendage TS16: embryo ectoderm TS16: embryo mesoderm TS16: head TS16: musculature TS16: musculoskeletal system TS16: respiratory system	Absent	0.1-1 µg; total RNA	BALB/c		Not Specified	embryo after dissection of the AGM (aorta, gonads, mesonephros, gut and mesentery), yolk sac and liver.
10.5	E11.0 (a)	TS18: appendage TS18: embryo ectoderm TS18: embryo mesoderm TS18: head TS18: musculature TS18: musculoskeletal system TS18: respiratory system	Absent	0.1-1 µg; total RNA	BALB/c		Not Specified	embryo after dissection of the AGM (aorta, gonads, mesonephros, gut and mesentery), yolk sac and liver.
11	E11.5 (a)	TS19: embryo	Present	0.1-1 µg; total RNA	BALB/c		Not Specified	embryo after dissection of the AGM (aorta, gonads, mesonephros, gut and mesentery), yolk sac and liver.
12	E12.5 (a)	TS20: embryo	Present	0.1-1 µg; total RNA	BALB/c		Not Specified	embryo after dissection of the AGM (aorta, gonads, mesonephros, gut and mesentery), yolk sac and liver.
13	E13.5 (a)	TS21: embryo	Present	0.1-1 µg; total RNA	BALB/c		Not Specified	embryo after dissection of the AGM (aorta, gonads, mesonephros, gut and mesentery), yolk sac and liver.

Notes:
(a) Age normalized so that noon of plug day = E0.5.

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