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Gene Expression Data
Assay Details
Assay
Reference: J:39086 Marcos MA, et al., Antigenic phenotype and gene expression pattern of lymphohemopoietic progenitors during early mouse ontogeny. J Immunol. 1997 Mar 15;158(6):2627-37
Assay type: RT-PCR
MGI Accession ID: MGI:2670411
Gene symbol: Rag1
Gene name: recombination activating 1
Probe: F3, R6
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The RT-PCR reaction was electrophoresed, Southern blotted and hybridized with a probe (MGI:2670373). The data is presented in Figure 5A in bar graph format of the densitometric quantification of autoradiographic band relative to control band.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 E12.5 (a) TS20: blood Present 0.1-1 µg; total RNA BALB/c Not Specified peripheral blood cells
Lane 2 E13.5 (a) TS21: blood Present 0.1-1 µg; total RNA BALB/c Not Specified peripheral blood cells
Lane 3 E12.5 (a) TS21: lesser omentum Not Specified 0.1-1 µg; total RNA BALB/c Not Specified
Lane 4 E13.5 (a) TS21: lesser omentum Present 0.1-1 µg; total RNA BALB/c Not Specified
Lane 5 E14.5 (a) TS22: lesser omentum Present 0.1-1 µg; total RNA BALB/c Not Specified
Lane 6 E12.5 (a) TS20: liver Present 0.1-1 µg; total RNA BALB/c Not Specified
Lane 7 E13.5 (a) TS21: liver Present 0.1-1 µg; total RNA BALB/c Not Specified
Lane 8 E14.5 (a) TS22: liver Present 0.1-1 µg; total RNA BALB/c Not Specified
Notes:
(a) Age normalized so that noon of plug day = E0.5.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/19/2024
MGI 6.24
The Jackson Laboratory