Antibody:	anti-Hoxb4
Assay notes:	Authors state assay was performed as previously described by Gould et al., 1997 (J:39637) who report using 4% formaldehyde.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 7	Present		3A , 3B	Expression in the neural tube up to the rhombomere 6/7 boundary.
TS17: rhombomere 8	Present		3A , 3B	Expression in the neural tube up to the rhombomere 6/7 boundary.
TS17: neural tube	Present	Regionally restricted	3A , 3B	Expression in the neural tube up to the rhombomere 6/7 boundary.
TS17: trunk paraxial mesenchyme	Present	Regionally restricted	3B , 3A	Expression in the paraxial mesoderm up to somite 7.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	6E	Expression only detectable in posterior hindbrain.
TS17: neural tube	Present	Regionally restricted	6E	There is no expression in more posterior regions of the neural tube, even though expression is present in more anterior portions of the neural tube.
TS17: somite 07	Present		6E	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 08	Present		6E	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 09	Present		6E	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 10	Present		6E	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 11	Present		6E	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 12	Present		6E	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 13	Present		6E	Expression in somites 7 to 13, but not in more posterior somites.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	6F	Expression only detectable in posterior hindbrain.
TS17: neural tube	Present	Regionally restricted	6F	There is no expression in more posterior regions of the neural tube, even though expression is present in more anterior portions of the neural tube.
TS17: trunk paraxial mesenchyme	Present	Regionally restricted	6F	Expression in the paraxial mesoderm up to somite 7.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	6G	Expression only detectable in posterior hindbrain.
TS17: neural tube	Present		6G	There is no expression in more posterior regions of the neural tube, even though expression is present in more anterior portions of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: tail	Absent		6H

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	6K	There is no expression in more posterior regions of the neural tube, even though expression is present in more anterior portions of the neural tube.
TS17: trunk mesenchyme	Present		6K	Expression in all tissue surrounding the neural tube.
TS17: trunk dermomyotome	Strong		6K	Noticeably higher expression in the dermomyotome.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	6L	There is no expression in more posterior regions of the neural tube, even though expression is present in more anterior portions of the neural tube.
TS17: trunk somite	Present	Regionally restricted	6L	Although expression was seen at more anterior levels, no expression was detected in somites at this level.
TS17: nephric duct, mesonephric portion	Present		6L	Authors report expression in notochord and mesonephric duct.
TS17: notochord	Present		6L	Authors report expression in notochord and mesonephric duct.