Probe:	Hoxb4 probe9
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Authors state assay was performed as previously described by Summerbell et al., 2000 (J:63685) who report using Mirsky's fixative.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		6A	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb
TS17: rhombomere 7	Present		6A	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb
TS17: rhombomere 8	Present		6A	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb
TS17: somite 07	Present		6A	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 08	Present		6A	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 09	Present		6A	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 10	Present		6A	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 11	Present		6A	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 12	Present		6A	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 13	Present		6A	Expression in somites 7 to 13, but not in more posterior somites.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		6B	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb region.
TS17: rhombomere 7	Present		6B	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb region.
TS17: rhombomere 8	Present		6B	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb region.
TS17: somite 07	Present		6B	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 08	Present		6B	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 09	Present		6B	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 10	Present		6B	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 11	Present		6B	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 12	Present		6B	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 13	Present		6B	Expression in somites 7 to 13, but not in more posterior somites.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		6C	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb region.
TS17: rhombomere 7	Present		6C	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb region.
TS17: rhombomere 8	Present		6C	Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb region.
TS17: somite 07	Present		6C	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 08	Present		6C	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 09	Present		6C	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 10	Present		6C	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 11	Present		6C	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 12	Present		6C	Expression in somites 7 to 13, but not in more posterior somites.
TS17: somite 13	Present		6C	Expression in somites 7 to 13, but not in more posterior somites.

Structure	Level	Pattern	Image	Note
TS17: tail	Strong		6D

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	6I	Expression throughout the neural tube, clearly stronger in the dorsal region.
TS17: trunk mesenchyme	Present		6I	Expression in all tissue surrounding the neural tube.
TS17: trunk dermomyotome	Strong		6I	Noticeably higher expression in the dermomyotome.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		6J
TS17: trunk somite	Present	Regionally restricted	6J	Although expression was seen at more anterior levels, no expression was detected in somites at this level.